Nā huahana

ʻO ka maʻi o ka maʻi momona non-alcoholic (NAFLD) he ākea ākea o nā mokuʻāina mai ka steatosis maʻalahi i hōʻike ʻia e ka paʻa ʻana o nā lipids i loko o nā pūnaʻi ate a hiki i ka steatohepatitis (NASH) e hōʻike ana i ka hōʻiliʻili momona, ʻā, ballooning a me ka degradation o nā hepatocytes, a me ka fibrosis.ʻO ka hope loa, hiki i ka steatohepatitis ke hopena i ka ate cirrhosis a me ka maʻi hepatocellular carcinoma. Ma kēia noiʻi ʻana mākou i kālailai i ʻekolu mau ʻano ʻiole like ʻole, A/J, C57BL/6J, a me PWD/PhJ, e hōʻike ana i nā degere like ʻole o ka steatohepatitis ke lawelawe ʻia kahi 3,5-diethoxycarbonyl. -1,4-dihydrocollidine (DDC) loaʻa ka meaʻai.RNA-Seq gene expression analysis, protein analysis and metabolic profiling i hoʻohana ʻia no ka ʻike ʻana i nā genes/proteins i hōʻike ʻokoʻa ʻia a me nā pae metabolite perturbed o nā ʻiole ate ma luna o ka DDC-treatment.Hōʻike ʻia ka loiloi ala i ka hoʻololi ʻana o ka waikawa arachidonic (AA) a me ka metabolism S-adenosylmethionine (SAMe) ma nā ala ʻē aʻe.No ka hoʻomaopopo ʻana i nā hoʻololi metabolic o ka metabolism arachidonic acid i ka mālamalama o nā profile hōʻike maʻi, ua hoʻomohala ʻia kahi ʻano kinetic o kēia ala a hoʻopaʻa ʻia e like me nā pae metabolite.Ma hope mai, ua hoʻohana ʻia ke kumu hoʻohālike e aʻo ai i nā hopena silico o nā pahuhopu lāʻau kūpono no ka steatohepatitis. Ua ʻike mākou i ka metabolism AA / eicosanoid i pilikia nui i nā ʻiole DDC-induced me ka hoʻohana ʻana i kahi hoʻokolohua hoʻokolohua a me ke ʻano silico.ʻO kā mākou hōʻuluʻulu ʻana i ka AA/eicosanoid metabolic pathway e hōʻike ana i ka 5-hydroxyeicosatetraenoic acid (5-HETE), 15-hydroxyeicosatetraenoic acid (15-HETE) a me ka prostaglandin D2 (PGD2) i pilikia i nā ʻiole DDC.Hōʻike hou mākou e hiki ke hoʻohana ʻia kahi hiʻohiʻona ikaika no ka wānana qualitative o nā loli metabolic e pili ana i ka ʻikepili transcriptomics i kahi pōʻaiapili pili i ka maʻi.Eia kekahi, ua ʻike ʻia ʻo SAMe metabolism ma muli o ka mālama ʻana o DDC.Hōʻike kekahi mau genes a me kekahi mau metabolites o kēia module i nā ʻokoʻa ma waena o A/J a me C57BL/6J ma kekahi ʻaoʻao a me PWD/PhJ ma kekahi.