Nā huahana

Ua hoʻohana ʻia kahi palena ITP counter-balanced EOF no ka hoʻopaʻa ʻana i nā anion mai nā laʻana conductivity kiʻekiʻe i ka wā hoʻomau electrokinetic injection o ka hāpana.I loko o ka polystyrenesulfonate/poly(diallyldimethylammonium chloride) polyelectrolyte coated capillary, hiki ke hoʻolōʻihi ʻia ka manawa i puka ai ka palena ITP i ka capillary ma ke kaulike ʻana i ka neʻe ʻana o ka palena me ka EOF.Ke hoʻohana nei i kahi electrolyte bis-tris-propane, ua wehe ʻia ka palena ITP mai ka capillary i loko o 7min, ʻoiai i ka wā e hoʻohana ai i ka triethanolamine aia nō ka palena ITP ma 30% o ka capillary ma hope o 2h o ka injection.Ke hoʻohana nei i kēia mau ʻōnaehana, ua hoʻomaikaʻi ʻia ka naʻau o ka hui ʻana o nā waikawa organik maʻalahi i 100mM Cl(-) e 700-800-fold me ka hoʻohana ʻana i ka bis-tris-propane me kahi hoʻoheheʻe capillary holoʻokoʻa o ka hāpana a me 5min o ka electrokinetic injection ma +28kV , a me 1100-1300-fold e hoʻohana ana i ka triethanolamine a me 60min o ka electrokinetic injection ma lalo o nā kūlana like.Ua hōʻike ʻia ka hiki o ke ala e pili ana i nā laʻana conductivity kiʻekiʻe ma o ka hoʻopaʻa ʻana i kahi capillary holoʻokoʻa i hoʻopiha ʻia me ka mimi i hoʻopaʻa ʻia me ka waika naphthalenedisulfonic, me nā palena o ka ʻike ʻana he 450 mau manawa haʻahaʻa ma mua o nā mea hiki ke loaʻa me kahi injection hydrodynamic maʻamau.