2′-Deoxythymidine Cas: 50-89-5
| Helu Helu | XD90582 |
| inoa mea kūʻai | 2'-Deoxythymidine |
| CAS | 50-89-5 |
| ʻĀpana Molekala | C10H14N2O5 |
| Kaumaha Molecular | 242.23 |
| Nā kikoʻī mālama | Ambient |
| Kānāwai Kūʻai Kūlike | 29349990 |
Huahana Huahana
| Ka nana aku | ʻO ka pauka keʻokeʻo keʻokeʻo |
| Hoʻāʻo | 99% |
| Nalo ma ka maloo | <0.5% |
| Koena ma ka Ignition | <0.3% |
| ʻO ka hoʻololi ʻana i ka optical kikoʻī | +17.5 - +19.5 |
Ua ʻike mua mākou i nā kaʻina hoʻoponopono kahi i hoʻohana ai ka dihydrotestosterone (DHT) i kāna hopena pale i ka parathyroid hormone-related protein (PTHrP) gene repression ma o ka estrogen receptor (ER)α, akā ʻaʻole ka androgen receptor (AR), i ka maʻi maʻi umauma MCF-7 cell. .Ma ʻaneʻi, ua noiʻi mākou inā loaʻa kēlā ʻano aberrant ligand-nuclear receptor (NR) i loko o nā cell LNCaP maʻi prostate.ʻO ka mea mua, ua hōʻoia mākou ua hōʻike nā pūnaewele LNCaP i ka nui o AR ma nā pae haʻahaʻa o ERα / β a i ʻole progesterone receptor.ʻO ka hoʻopau ʻana i ka PTHrP a me ka hoʻōla ʻana i nā genes antigen kikoʻī prostate i nānā ʻia ma hope o ka hoʻokele kūʻokoʻa o 17β-estradiol (E2), DHT, a i ʻole R5020.E like me ka manaʻo ua nalowale ka LNCaP AR i kāna ligand kikoʻī ma muli o kahi mutation (Thr-Ala877), nā hoʻokolohua me siRNA e kuhikuhi ana i kēlā me kēia NR i hōʻike ʻia ua hoʻokaʻawale ʻia ka AR i ka hana o ka mea hoʻoponopono o ka hoʻoponopono pili i ka hormone, i pili mau. me ka nukili translocatio n o keia mutant AR.Ua hōʻike ʻo Microarray analysis o ka hoʻoponopono gene e DHT, E2, a i ʻole R5020 ua ʻoi aku ma mua o ka hapalua o nā genes i lalo o ka AR (Thr-Ala877) i uhi ʻia i loko o nā pūnaewele LNCaP.ʻO ka hoihoi nui, ua ʻike mākou he kuleana like ka AR (wild-type [wt]) a me AR (Thr-Ala877) no nā pilina E2-AR.Ua hōʻike ʻia nā hoʻokolohua microscopy fluorescence ʻo EGFP-AR (wt) a me EGFP-AR (Thr-Ala877) i hoʻopili wale ʻia i loko o ka nucleus ma hope o ka mālama ʻana iā E2 a i ʻole DHT.Eia kekahi, ua hōʻike ʻia nā mea hōʻike hōʻike e hōʻike ana kekahi mau maʻi maʻi maʻi ʻē aʻe i ka hōʻailona E2-AR (wt) aberrant e like me ka mea i loko o nā cell LNCaP.Ke hoʻopuka nei mākou i ka hiki ʻana o nā pilina pili i waena o wt AR a me E2 i kekahi mau ʻano maʻi maʻi maʻi hormone-sensitive.
