Nā huahana

ʻO 1.Optically active D-2-hydroxybutanoate he mea koʻikoʻi hale kūkulu waena no nā lāʻau lapaʻau a me ka polydegradable poly(2-hydroxybutanoate).ʻO ka hoʻonā kinetic o racemic 2-hydroxybutanoate he ʻōmaʻomaʻo a makemake ʻia no ka hana D-2-hydroxybutanoate.Ma kēia hana, D-2-hydroxybutanoate ma kahi kiʻekiʻe kiʻekiʻe (0.197 M) a me kahi kiʻekiʻe enantiomeric kiʻekiʻe (99.1%) i hana ʻia e kahi NAD-kūʻokoʻa L-lactate dehydrogenase (L-iLDH) i loaʻa ka biocatalyst.ʻO 2-Oxobutanoate, kekahi mea koʻikoʻi koʻikoʻi, i hana pū ʻia ma kahi kiʻekiʻe kiʻekiʻe (0.193 M).Ke hoʻohana nei i kahi kaʻina hana hoʻololi ion maʻalahi me ka macroporous anion exchange resin D301, ua hoʻokaʻawale ʻia ʻo D-2-hydroxybutanoate mai ka ʻōnaehana biotransformation me ka hoʻihoʻi kiʻekiʻe o 84.7%.

2. Ua hōʻike ʻia ka synthesis asymmetric o kahi waikawa amino kūlohelohe e ka omega-transaminase mai Vibrio fluvialis JS17.L-2-Aminobutyric acid ua synthesized mai 2-oxobutyric acid a me benzylamine me ka enantiomeric oi aku ma mua o 99%.Ua hōʻike ʻia ka hopena i ka pale ʻana i ka huahana e benzaldehyde, i lanakila ʻia ma ka hoʻohana ʻana i kahi ʻōnaehana hoʻololi biphasic e wehe i ka huahana pale mai ka pae wai.Ma ka hopena biphasic maʻamau (50 mM 2-oxobutyric acid, 70 mM benzylamine a me 2.64 U/ml hoʻomaʻemaʻe enzyme) me ka hoʻohana ʻana i ka hexane ma ke ʻano he extractant, hiki i ka hoʻololi ʻana o 2-oxobutyric acid i 96% i ka 5 h akā ʻo 39% wale nō ka hoʻololi ʻana i loaʻa me ka ʻole. ka unuhi huahana.