Nā huahana

Ua hoʻomohala ʻia kahi aptasensor kūpono kahi i hoʻohana ʻia ai ka graphene oxide (GO) ma ke ʻano he hōʻailona no ka spectroscopy impedance electrochemical a me ka hana hōʻailona electrochemiluminescence (ECL).Ua hana ʻia ka aptasensor ma o ka hoʻohui ʻana iā ia iho i ka ʻimi ECL o kahi adenosine triphosphate binding aptamer (ABA) i hoʻopaʻa ʻia me kahi Ru complex (Ru(bpy)3(2+) derivatives) ma luna o ka ʻili o ke kalapona aniani gula (AuNP) i hoʻololi ʻia. electrode (GCE).ʻO ka ABA i hoʻopaʻa ʻia ma ka AuNP i hoʻololi ʻia ʻo GCE hiki ke hoʻopili ikaika iā GO ma muli o ka pilina ikaika π-π ma waena o ABA a me ka graphene oxide;ʻO ka hoʻopau ʻana o ka ECL o ka Ru complex ma muli o ka hoʻololi ʻana o ka ikehu a me ka hoʻololi ʻana o ka electron, a me ka piʻi nui ʻana o ke kūʻē electron transfer (Ret) o ka electrode.ʻOiai ma ke alo o ka target adenosine triphosphate (ATP), makemake ka ABA e hana i ka ABA-ATP bioaffinity complexes, kahi nāwaliwali nāwaliwali i ka graphene oxide a mālama i ka graphene oxide mai ka ʻili electrode, no laila e ʻae i ka hoʻonui ʻana i ka hōʻailona ECL, a i hui pu me ka emi ana o ka Ret.Ma muli o ke kiʻekiʻe o ka ECL quenching efficiency, kū hoʻokahi, a me nā waiwai uila o ka graphene oxide, ʻo ka Ret a me ka ECL ikaika e kūʻē i ka logarithm o ka ʻike ATP ua linear ma ka laulā ākea mai 10 pM a i 10 nM me ka palena ʻike haʻahaʻa loa o 6.7 pM a 4.8 pM, kēlā me kēia.Ua hōʻike ʻia ka aptasensor i manaʻo ʻia i ka reproducibility maikaʻi loa, kūpaʻa, a me ke koho koʻikoʻi, a hiki ke hoʻokaʻawale maikaʻi ʻia ka ATP mai kāna mau analogues.ʻOi aku ka nui, ʻo kēia hoʻolālā ECL aptasensor maikaʻi e pili ana iā GO e hana ana ma ke ʻano he electrochemical a me ECL hōʻailona hōʻailona maʻamau a hiki ke hoʻonui maʻalahi i nā hanana paʻa olaola ʻē aʻe.