Nā huahana

Chemifluorescence: ʻO ka luminol molekula he mole kemika fluorescent hiki ke hoʻololi ʻia i ʻakika aminophthalic state hauʻoli i ke alo o nā molekole hydrogen peroxide, e hoʻopuka ana i ka fluorescence ikaika.ʻO ka hydrogen peroxide ka huahana o nā hopena biooxidative he nui, no laila ua maʻalahi ka hoʻopili ʻana i kēia mau hopena biooxidative me ka photodetection ma ka hoʻokomo ʻana i ka luminol.No ka laʻana, hiki i ka glucose oxidase/catalase probe ke ʻike i ka neʻe ʻana o ka hydrogen peroxide a i ʻole ka glucose i loko o ka hāpana, a ʻo ka manawa pane he 0.5s wale nō (ka hana hoʻoikaika).ʻO ka hui pū ʻana i nā mea chemiluminescent me nā hopena immunological, a me ka hōʻike ʻana i ka ʻike ʻana o nā ʻāpana immune i ʻike ʻia e ka māmā māmā, ua kapa ʻia ʻo chemiluminescent immunoassay technology.I ka makahiki 1976, ua noi ʻo Shmeder i ka chemiluminescence immunoassay, me ka hoʻohana ʻana i ka luminol-H202 a me kāna derivative ABEI ma ke ʻano he ʻōnaehana hōʻike ʻike.I kēia manawa, ʻo ka luminol a me kāna mau derivatives ka mea i hoʻohana pinepine ʻia i nā noi chemiluminescence immunoassay.Ma lalo o nā kūlana alkaline, catalyzed e microperoxidase, hiki ke hoʻokuʻu ʻia ka nui o nā photons.Hiki ke hoʻohana ʻia ʻo Luminol no WesternBlot o nā antibodies i hoʻopaʻa ʻia i ka HRP a me ka ʻike ʻana i ka hybridization nucleic acid o nā probes-labeled HRP.Hoʻohana ʻia ia i ka ʻike koko koko.ʻO kahi pūhui i hiki ke hoʻohana ʻia e like me ka marker chemiluminescent pono e hoʻokō i kēia mau kūlana: kiʻekiʻe ka hua nui o ka luminescence;ua kūlike kona mau waiwai physicochemical me ka ʻōnaehana i aʻo ʻia;ʻO kona hopena luminescent ka hopena o ka hopena oxidation o ka mea luminescent;i loko o ka pae hoʻonaʻauao i hoʻohana ʻia.Hoʻohana mau ʻia kekahi mau ʻano o ka chemiluminescence reagents: acridine ester, he mea hoʻohana nui chemiluminescence tracer, he tricyclic organic compound, maʻalahi e oxidize, a ʻaʻole pono ka hopena oxidation i ka catalyst, a hoʻokuʻu i nā photons ma 430nm;ʻO Luminol a me isoluminol a me kā lākou mau mea hoʻoheheʻe ʻo ia nā mea hana chemiluminescent oo loa.Ma mua o 1964, ʻOiai i hōʻike ʻia ma ka chemiluminescence, ua hoʻohana ʻia ka luminol, isoluminol a me kā lākou derivatives no chemiluminescence.Ua loaʻa i nā Immunoassay nā hopena maikaʻi aʻe.Ka ʻike ʻana o ka luminescence: ʻo ka lōʻihi o ka nalu fluorescence ʻo 400nm (ka ʻike ʻana o chemiluminescence ma 60mmK2S2O8, 100mmK2CO3, pH11.5 solution)

Chemiluminescence: Ma ke ʻano he ʻano hoʻokolohua koʻikoʻi, hoʻohana nui ʻia ka chemiluminescence e ʻike i nā radical manuahi a me nā metabolites hopena i hana ʻia i loko o nā enzymes, cell a me nā mea olaola.Hiki ke hoʻohana ʻia ka māmā e kā lākou huahana oxidation a me nā metabolites me nā photometers like ʻole.ʻike maka.Hoʻohana nui ʻia ka Chemiluminescence i ka nānā ʻana a me ka noiʻi ʻana o nā antioxidants, e like me polyphenols, polysaccharides, flavonoids, a me anthraquinones, no kona ʻano naʻau, wikiwiki, hana maʻalahi a me ke kumu kūʻai haʻahaʻa.ʻO nā ʻōnaehana chemiluminescence maʻamau i hoʻohana ʻia no ka hoʻoholo ʻana i nā anion superoxide he xanthine oxidase-luminol, pyrogallol-luminol a me dimethyl sulfoxide-luminol.ʻO ka mua he ʻōnaehana enzymatic, a ʻo nā mea hope ʻelua He ʻōnaehana non-enzymatic.ʻO nā ʻōnaehana chemiluminescence no ka hoʻoholo ʻana i nā radical hydroxyl ka nui o ka copper sulfate-yeast (a i ʻole nā ​​​​pūnaewele iwi iwi) -ascorbic acid-hydrogen peroxide, CuCl-H2O2-o-phenanthroline-carbonate buffer, copper sulfate-o-phenanthroline-ascorbic acid-hydrogen peroxide 5 chemiluminescence system, ferrous sulfate-luminol-hydrogen peroxide a me ferrous sulfate-luminol, e pili ana i ka hopena Fenton maʻamau e hoʻoulu i nā radical hydroxyl, a laila e hoʻouka i ka mea luminescent e hoʻohua i ka chemiluminescence, hiki ke hoʻohana ʻia e ana i ka wehe ʻana i nā extracts. ka hana o nā radical oxygen reactive.ʻO ke ʻano hoʻokolohua: hoʻohana i ka pyrogallol-luminol chemiluminescence system: hana ʻia ka luminol i 0.05mol/L ka hoʻonā ʻana me 0.05mol/L NaOH solution, mālama ʻia i kahi pouli, a me ka wai distilled pālua ma mua o ka hoʻohana ʻana E hoʻoheheʻe i ka solution 1mmol/L, hoʻohana i ka 1mmol/ L HCl e hana i ka 0.01mol/L solution o ka pyrogallol, e mālama i loko o ka pahu hau ma 4°C, a e hoʻoheheʻe ʻia me ka wai distilled pālua i 16 manawa 6.25 × 10-4mol/L solution ma mua o ka hoʻohana ʻana.0.05mol/L pH10.2Na2CO3-NaHCO3 buffer (loa'a ka 0.1mmol/LEDTA) ma mua o ka ho'ohana 'ana, a hui 'ia me 1mmol/L luminol ma 2:1 (nui ka hapa) e hana i ka luminol a me ka carbonate buffer ma mua o ka hui 'ana.I ka wā o ke ana ʻana, e hoʻokomo i 10.0 μL mau laʻana o nā ʻokoʻa like ʻole (0, 0.08, 0.4, 2 a me 10 mg/mL) i loko o ka luminescent cell (me ka laʻana buffer e like me ka mana), a laila hoʻokomo i ka 6.25 × 10-4mol/L pyrogallol. 0.05 ʻO ka hope, ua hoʻohui ʻia ka 0.94 mL o ka hui ʻana o luminol a me carbonic acid buffer no ka hoʻomaka ʻana i ka hopena (30°C), ua helu ʻia ka ikaika o ka luminescence ma nā manawa o 2 s, a ua ana ʻia ka nui o ka luminescence i hoʻohui ʻia ma 300 s.ʻO ka ikaika o ka luminescence hope ka luminescence ke hoʻohui ʻole ʻia ka pyrogallol.waiwai.Eia kekahi, aia nā chemiluminescence, fluorescence spectroscopy a me NBS-dichlorofluorescein chemiluminescence system no ka hoʻoholo ʻana i ka fluorescence chemiluminescence, etc.

Nā Waiwai Kemika: Ka pauda crystalline melemele.Hiki ke hoʻoheheʻe ʻia i ka lye, hiki ke hoʻoheheʻe ʻia i loko o ka waiʻona dilute, aneane hiki ʻole ke hoʻoheheʻe ʻia i ka wai, a hiki ʻole ke hoʻoheheʻe ʻia i ka waiʻona.Hōʻike nā haʻina kūʻokoʻa a i ʻole ʻakika liʻiliʻi i ka fluorescence polū ʻālohilohi ke ʻike ʻia i ke kukui ultraviolet.Ka helu hehee 329-332 ℃

Hoʻohana: Ma ke ʻano he reagent a me ka hōʻailona kemika.E hoʻohana i ka Chemiluminescence analysis detection reagent (e like me ka hoʻoholo ʻana i nā cation metala a i ʻole ke koko)

Hoʻohana: No ka nānā ʻana i nā chemiluminescence, e like me: cation metala, koko a me nā glucocorticoids

Hoʻohana: Luminescence hōʻike: Emmax440nm (chemiluminescence; 60mmK2S2O8, 100mmK2CO3, pH11.5; ma hope o ka hoʻohui ʻana i ka H2O2) chemiluminescence reagent a me ka hōʻailona, ​​hoʻohana mau ʻia i ka nānā ʻana i ka chemiluminescence, e like me nā cations metala, ka pale koko, etc.