Nā huahana

Nā hopena o nā mea hoʻomaʻemaʻe ma ka hana P-glycoprotein atpase: nā ʻokoʻa i nā pilikia o nā hana basal a me verapamil.

ʻO P-glycoprotein (P-gp), he glycoprotein membrane plasma e pili ana me ka phenotype resistance multidrug, ke kuleana o ka efflux hilinaʻi ATP o nā lāʻau amphiphilic like ʻole.Me ka hoʻohana ʻana i nā vesicle membrane i hoʻomākaukau ʻia mai ka multidrug resistant cell line DC-3F/ADX, ua aʻo mākou i ka perturbation o ka basal (ʻo ia hoʻi me ka loaʻa ʻole o ka lāʻau lapaʻau) a me nā hana P-gp ATPase hilinaʻi verapamil i hoʻoulu ʻia e nā mea holoi like ʻole, i ka non-solubilizing, e like me ka hoʻoheheʻe ʻana, ka hoʻopaʻa ʻana.Ua nānā ʻia ka holomua o ka membrane solubilization me ka hoʻonui ʻana i ka detergent e ka hoʻopuehu māmā a me nā hoʻokolohua centrifugation.No ka hoʻoheheʻe ʻole ʻana i ka mea hoʻomaʻemaʻe, ʻo nā mea holoi hoʻāʻo a pau koe wale nō DOC i hoʻokomo i kahi kaohi ʻana o ka hana P-gp ATPase, ʻaʻole i hoʻopili ʻia me ka nui o nā mea holoi hoʻāʻo like ʻole i hoʻohui ʻia i loko o nā membrane.Hōʻike ka nānā ʻana o ka verapamil-induced P-gp activation i ka hoʻololi ʻokoʻa ʻana o ka hana P-gp ATPase e nā mea holoi like ʻole i ka non-solubilizing concentrations.No laila,ʻoi aku ka nui o nā pilina ma waena o P-gp a me nā mea holoi ma mua o ka hoʻopiʻi membrane honua.Ma hope o ka hoʻoheheʻe ʻia ʻana e nā mea hoʻomaʻemaʻe i hoʻāʻo ʻia, ua ʻoki loa ʻia ka hana basal P-gp ATPase, koe wale nō ke alo o CHAPS i hiki ke mālama i kēia hana ma kahi pae e hoʻohālikelike ʻia me ka mea i ana ʻia i loko o nā membrane maoli.Eia naʻe, ua nalowale ka verapamil-induced P-gp ATPase activation i ka wā P-gp solubilization e CHAPS, akā ua hoʻihoʻi hou ʻia ma hope o ka hoʻoheheʻe ʻana o CHAPS ma lalo o kāna ʻano micellar koʻikoʻi.Hōʻike kēia mau ʻike i nā pilina kikoʻī ma waena o nā molekole P-gp a me CHAPS i loko o nā micelles hui ʻia.Ma ka holoʻokoʻa, ʻo kā mākou ʻikepili e hōʻike ana i nā pilina kikoʻī P-gp / detergents e kūlike me ka wahi o nā wahi lawe lāʻau lapaʻau ma nā kikowaena transmembrane P-gp.