4-Aminophthalhydrazide AMPPD Cas:3682-14-2 Melemele māmā i amber i ka pauka ʻōmaʻomaʻo ʻeleʻele i ka puʻupuʻu.
| Helu Helu | XD90156 |
| inoa mea kūʻai | 4-Aminophthalhydrazide |
| CAS | 3682-14-2 |
| ʻĀpana Molekala | C8H7N3O2 |
| Kaumaha Molecular | 177.16 |
| Nā kikoʻī mālama | Hale kūʻai ma RT |
Huahana Huahana
| Ka nana aku | ʻO ka melemele māmā i ka amber a hiki i ka pauka ʻōmaʻomaʻo ʻeleʻele i ka puʻupuʻu |
| ʻO Assay | ≥98.0% |
| ʻO ka mānoanoa | 1.433 |
| Lae hehee | 300 ºC |
| Lae paila | 633°C ma 760 mmHg |
| Kuhikuhi uila | 336.7°C |
| Ka hikiwawe | Hiki ke hoʻoheheʻe ʻia i loko o ka ammonium hydroxide, sodium carbonate a i ʻole kumu ʻē aʻe. |
1.We kālailai a hoʻohālikelike i ka hopena o ʻelima H1-antihistamines ma stimulated oxidative burst ma extra- a intracellular pae o ka kaawale a hoʻoulu kanaka polymorphonuclear leukocytes.Oxidative burst o kaawale kanaka neutrophils ua aʻo 'ia e ka luminol a me isoluminol hoʻonui chemiluminescence. ʻO ka hoʻonohonoho kūlana o ka mana no ka H1-antihistamines e hoʻemi i ka chemiluminescence ua loiloi extracellularly: dithiaden> loratadine> chlorpheniramine> brompheniramine> pheniramine a ma ka pūnaewele intracellular: loratadine> dithiaden. ʻOkoʻa nui nā H1-antihistamines e like me kā lākou hana kemika i ka hoʻopau ʻana i ka oxidative burs. - a me ka pūnaewele intracellular o nā neutrophils kanaka i hoʻokaʻawale ʻia.
ʻO nā ʻōnaehana 2.Chemiluminescence i hoʻonui ʻia e ka isoluminol a i ʻole luminol i hui pū ʻia me kahi peroxidase nā ʻano maʻalahi no ka ʻike ʻana i nā ʻano oxygen reactive (ROS) i hana ʻia e phagocyte NADPH oxidase.ʻO nā substrate hoʻonui ʻelua he ʻano like loa, ʻokoʻa wale nō ke kūlana o ka pūʻulu amino i ke apo ʻala o nā mole.Hoʻololi kēia ʻokoʻa i ka isoluminol i lipophilic liʻiliʻi liʻiliʻi i emi ʻole i nā membrane biological.No laila ua kaupalena ʻia ka hoʻohana ʻana o isoluminol i nā haʻawina e pili ana i ka huna ʻana o nā metabolites oxygen.Ma kēia haʻawina, hōʻike mākou i nā peptides synthetic i loaʻa mai ka N-terminal domain o ka calcium-regulated protein annexin AI e hoʻopilikia i ka ʻike ʻana o nā radical i kahi isoluminol-amplified, akā ʻaʻole i loko o kahi luminol-amplified, ʻōnaehana.ʻO nā peptides i loaʻa i ka annexin AI e hoʻemi i ka puka māmā me ka isoluminol i hauʻoli e ka superoxide a me ka horseradish peroxidase (HRP) i ka formyl-methionyl-leucyl-phenylalanine- a me ka phorbol koʻu ristate acetate-stimulated cell, a me ka hydrogen peroxide a me HRP.ʻAʻole ʻike ʻia ke ʻano kikoʻī no ka hana ʻana.Hōʻike ikaika ʻia nā hopena i hōʻike ʻia e hoʻopaʻa ʻia kahi pane kelepona hoʻemi ʻia me ka isoluminol-amplified chemiluminescence me kahi ʻano hana ʻē aʻe e hoʻoholo ai i ka hoʻokuʻu ʻana o nā anion superoxide a me ka hydrogen peroxide.
