Nā huahana

ʻO nā ana o ka alpha-amylase me 4-nitrophenyl glucosides e hāʻawi i kēia mau mea maikaʻi ma mua o nā ʻano hana e hilinaʻi nei i ka hoʻokumu ʻana o NADH: kahi manawa pōkole pōkole, ʻaʻohe ʻike ʻia e nā metabolites a me nā enzymes o ka laʻana a me nā substrates kūpaʻa loa me nā waiwai haʻahaʻa haʻahaʻa.Ua like ka naʻau intrinsic o ka hoʻokumu ʻana o nitrophenol me ka hydrolysis o maltotetraose, akā ʻoi aku ka liʻiliʻi ma mua o nā ʻano hana hana glucose me ka hoʻohana ʻana i ka oligosaccharides.He ʻokoʻa ka starch, ʻoi aku ka wikiwiki o ka hydrolysed o nā substrates chromogenic e ka salivary ma mua o ka amylase pancreatic.ʻO nā pōʻino o kēia mau substrates: ʻoi aku ka nui o ka huli ʻana e nā holoholona ma mua o nā amylases kanaka, a me ka maʻalahi o ka chromophore i nā hoʻololi liʻiliʻi o ka pH a me ka ʻike protein.ʻO kekahi mau hiʻohiʻona analytical e like me ka kikoʻī, pololei, pololei, kūpaʻa o ka substrate a me nā laina laina i wehewehe ʻia a hoʻohālikelike ʻia me nā ʻano hana ʻē aʻe.