4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4 Pada Keʻokeʻo Paʻa 98%
| Helu Helu | XD90011 |
| inoa mea kūʻai | 4-Nitrophenyl-alpha-D-mannopyranoside |
| CAS | 10357-27-4 |
| ʻĀpana Molekala | C12H15NO8 |
| Kaumaha Molecular | 30301.25 |
| Nā kikoʻī mālama | -2i -8°C |
| Kānāwai Kūʻai Kūlike | 29400000 |
Huahana Huahana
| Wai | <5% Karl Fische |
| Ka hikiwawe | 1% ma DMF he akaka a kala ʻole |
| Maemae | 4-Nitrophenol manuahi <200ppm |
| HPLC | >98% |
| Ka nana aku | pauda keʻokeʻo keʻokeʻo |
ʻO nā ʻike mīkini i loko o kahi ʻohana Ca2+ hilinaʻi o alpha-mannosidases i loko o kahi symbiont puʻuwai kanaka.
ʻO ka bacteria colonic, i hōʻike ʻia e Bacteroides thetaiotaomicron, he kuleana koʻikoʻi i ka mālama ʻana i ke olakino kanaka ma o ka hoʻohana ʻana i nā ʻohana nui o glycoside hydrolases (GHs) e hoʻohana i nā polysaccharides meaʻai a me nā glycans hoʻokipa ma ke ʻano he meaʻai.Hoʻohālikelike ʻia kēlā hoʻonui ʻohana GH e ka ʻohana 23 GH92 glycosidases i hoʻopili ʻia e ka B. thetaiotaomicron genome.Ma ʻaneʻi, hōʻike mākou he alpha-mannosidases kēia e hana nei ma o kahi ʻano hoʻoneʻe hoʻokahi e hoʻohana ai i nā host N-glycans.ʻO ka hoʻolālā ʻekolu-dimensional o ʻelua GH92 mannosidases e wehewehe i kahi ʻohana o nā protein ʻelua-domain kahi i loaʻa ai ka catalytic center ma ke kikowaena domain, e hāʻawi ana i ka waikawa (glutamate) a me ka base (aspartate) kōkua i ka hydrolysis i loko o kahi Ca (2+) - ʻano hilinaʻi.ʻO nā ʻano hana ʻekolu o nā GH92 i loko o ka paʻakikī me nā mea paʻa e hāʻawi i ka ʻike i ka kikoʻī, ka mīkini a me ka conformational itinerary o catalysis.He koʻikoʻi ko Ca(2+) i ke kōkua ʻana i ka hoʻokaʻawale ʻana i ka mannoside mai kona ʻano noho noho (4)C(1) i ke kūlana hoʻololi.（Bibliography: Nat.Kemi.Biola.6, 125-32, (2010)
ʻO ka chromatography pili mua o ka ovalbumin glycoasparagines ma kahi kolamu concanavalin A-sepharose.ʻO kahi noiʻi quantitative o ka paʻa ʻana o ka lectin.
ʻO nā pilina o Sepharose 4B-immobilized concanavalin A (ConA) me 10 glycoasparagines i loaʻa mai ka ovalbumin ua noiʻi nui ʻia e ka chromatography affinity frontal.Ma kēia ʻano hana, hoʻohana mau ʻia kahi hopena carbohydrate i kahi kolamu ConA-Sepharose a ua ana ʻia ka hoʻihoʻi ʻana o ka mua elution e like me ke ʻano o ka ikaika o ka pilina.Hiki ke hoʻoholo ʻia ka hoʻokaʻawale mau (Kd) no kēlā me kēia saccharide me ConA.Ua hōʻike ʻia kahi loiloi o ka hoʻopaʻa ʻana o p-nitrophenyl-alpha, D-mannoside ʻaʻole e loli maoli nā waiwai paʻa o ConA ma hope o ka immobilization ma Sepharose 4B.Ua kapa ʻia kēlā me kēia ovalbumin glycoasparagines me ka tritium e ke ʻano methylation reductive no ka nānā ʻana.ʻO ka hoʻohālikelike ʻana i nā koina Kd i loaʻa, ua ʻike ʻia he ʻano like ʻole ka hoʻopaʻa ʻana o ConA me nā ʻokoʻa liʻiliʻi liʻiliʻi o ke kaulahao glycosyl.Hōʻike nā hualoaʻa e ʻike ana ʻo ConA i kahi ʻano kaulahao glycosyl kikoʻī, Man alpha 1-6(Man alpha 1-3) Man, kahi i hoʻokahi pūʻulu hydroxyl ma ke kūlana C-3 o ka mannose pili i ka C-6-linked.
