Ua aʻo ʻia ka biosynthesis o nā proteoglycans a me nā glycosaminoglycans i mua o ka p-nitrophenyl-xyloside me ka hoʻohana ʻana i kahi ʻōnaehana moʻomeheu cell ovarian granulosa mua.ʻO ka hoʻohui ʻana o ka p-nitrophenyl-xyloside i loko o ke ʻano moʻomeheu cell i hoʻonui ʻia ma kahi o 700% o ka hoʻohui ʻana o [35S] sulfate (ED50 ma 0.03 mM) i loko o nā macromolecules, i komo pū me nā kaulahao chondroitin sulfate manuahi i hoʻomaka ʻia ma ka xyloside a me nā proteoglycans maoli.ʻO nā kaulahao chondroitin sulfate manuahi i hoʻomaka ʻia ma ka xyloside ua aneane huna wale ʻia i loko o ke kikowaena.Ua emi iho ka nui mole o nā kaulahao chondroitin sulfate mai 40,000 a i 21,000 i ka hoʻonui ʻia ʻana o ka hui ʻana o [35S] sulfate, e hōʻike ana i ka hoʻonui ʻia ʻana o ka chondroitin sulfate i hoʻopilikia i ka hana maʻamau o ka hoʻopau ʻana i ke kaulahao glycosaminoglycan.Ua hōʻemi ʻia ka biosynthesis o ka heparan sulfate proteoglycans ma kahi o 50%, ma muli paha o ka hoʻokūkū ma ke kiʻekiʻe o ka UDP-sugar precursors.[35S] Ua pani ʻia ka hoʻohui ʻana o ka cycloheximide me ka hapalua manawa ma kahi o 2 mau hola ma ke alo o ka xyloside, ʻoiai inā ʻaʻole i loaʻa ka xyloside ma kahi o 20 min.Hōʻike ka ʻokoʻa i ka nui o ka huli ʻana o ka glycosaminoglycan synthesizing capacity ma ke ʻano holoʻokoʻa.ʻOi aku ka pōkole o ka nui o ka huli ʻana o ka glycosaminoglycan synthesizing hiki i nā cell granulosa ovarian ma mua o ka mea i ʻike ʻia i loko o nā chondrocytes, e hōʻike ana i ka mana pili o ka hana biosynthetic proteoglycan i ka hana metabolic holoʻokoʻa o nā cell.
