Nā huahana

ʻO ka C6 dicarboxylic acid, adipic acid, he mea koʻikoʻi kemika ma ka ʻoihana.ʻO ka hana biobased o ka adipic acid kahi mea e hoʻohiki ai i ke ala petrochemical o kēia manawa.Maʻaneʻi, hōʻike mākou i ka biosynthesis o adipic acid me ka hoʻohana ʻana i kahi ala hana i hoʻoulu ʻia e ka hoʻohuli ʻana o ka beta-oxidation o nā waikawa dicarboxylic.Aia ke ala biosynthetic i ka condensation o ka acetyl-CoA a me ka succinyl-CoA e hana i ka iwi kuamoʻo C6 a me ka hoʻemi hope ʻana, dehydration, hydrogenation, a me ka hoʻokuʻu ʻana o ka adipic acid mai kāna thioester.Ua hoʻāʻo mua ʻia ke ala i loko o ka vitro me nā enzymes ala i hoʻohui ʻia a laila hoʻokomo pono ʻia i loko o Escherichia coli no ka biosynthesis a me ka excretion o adipic acid i loko o ka moʻomeheu moʻomeheu.Ua hoʻonui ʻia ka titer hana ma kahi o 20-fold ma o ka hui pū ʻana o ka hoʻoulu ʻana i nā enzyme i kūpono i ka hoʻoulu ʻana i nā hopena synthetic a me ka hoʻonui ʻana i ka loaʻa o nā substrates condensation.Hōʻike kēia hana i ka biosynthesis pololei o ka adipic acid ma o ke ala synthetic non-natural, hiki ke hiki i kāna hana hou.