Nā huahana

ʻO nā reagents hou o Trinder he mau mea hoʻoheheʻe aniline hiki ke hoʻoheheʻe ʻia i ka wai i hoʻohana nui ʻia i nā hoʻokolohua diagnostic a me nā hoʻokolohua biochemical.Nui nā pono ma mua o nā reagents chromogenic maʻamau i ka hoʻoholo colorimetric o ka hana hydrogen peroxide.Paʻa paʻa nā reagents hou o Trinder e hoʻohana ʻia i nā ʻōnaehana ʻike pipeline hoʻokolohua.Ma ke alo o ka hydrogen peroxide a me ka peroxidase, ua hōʻike ʻia ka moʻolelo Trinder's reagent me ka 4- aminoantipyrine (4-AA) a i ʻole 3-methylbenzothiazole sulfonehydrazone (MBTH) i ka wā o ka hoʻohui ʻana o ka oxidative.Hoʻokumu i ka viole paʻa a i ʻole nā ​​kala uliuli.ʻO ka molar absorbance o ka wai i hui pū ʻia me MBTH he 1.5- 2 mau manawa kiʻekiʻe ma mua o ka wai i hui pū ʻia me 4-AA;akā naʻe, ʻoi aku ka paʻa o ka solution 4-AA ma mua o ka solution MBTH.ʻO ka substrate ua hoʻoheheʻe ʻia e kāna oxidase e hana i ka hydrogen peroxide.ʻO ka hydrogen peroxide e pili ana i ka hoʻopili substrate.No laila, hiki ke hoʻoholo ʻia ka nui o ka substrate e ka ulu ʻana o ke kala o ka hopena hoʻohui oxidative.Hiki ke hoʻohana ʻia ka glucose, ka waiʻona, acyl-CoA a me ka cholesterol e ʻike i kēlā mau substrates i hui pū ʻia me ka reagent a Trinder a me 4-AA.Aia he 10 mau mea hou a Trinder i loaʻa.Ma waena o nā reagents hou a Trinder, ʻo TOOS ka mea maʻamau i hoʻohana ʻia.Eia nō naʻe, no kahi substrate kikoʻī, pono ka hoʻāʻo ʻana i nā papa like ʻole o ka moʻolelo Trinder's reagents e hoʻomohala i ka ʻōnaehana ʻike maikaʻi loa.