Ammonium hexachloroiridate(IV) CAS:16940-92-4
| Helu Helu | XD90606 |
| inoa mea kūʻai | Ammonium hexachloroiridate(IV) |
| CAS | 16940-92-4 |
| ʻĀpana Molekala | H8Cl6IrN2 |
| Kaumaha Molecular | 441.012 |
| Nā kikoʻī mālama | Ambient |
Huahana Huahana
| Ka nana aku | ʻOliva ʻōmaʻomaʻo kristal |
| Hoʻāʻo | 99% |
ʻO ka pōʻino oxidative i nā waihona DNA ka hopena maʻamau i ka hoʻokumu ʻana i nā purine oxidized, ʻoi aku ka 7,8-dihydro-8-oxoguanine (8-oxoG) a me 7,8-dihydro-8-oxoadenine (8-oxoA), ʻo ka mea mua he punawai- ʻike ʻia ka ʻeha mutagenic.Ma muli o ka hiki ke hoʻololi ʻia ʻo 8-oxoG me ka hoʻohālikelike ʻia me nā kumu maʻamau, ua noiʻi ʻia ka hoʻokomo ʻana o kahi kumu i ka wā synthesis DNA e kū pono ana i kahi ʻano oxidized o 8-oxoG i loko o ka vitro.Ua mālama mua ʻia kahi maʻi synthetic i loaʻa i kahi lesion 8-oxoG hoʻokahi me nā mea hoʻokaʻawale ʻokoʻa hoʻokahi-electron a i ʻole ma lalo o nā kūlana oxygen singlet a laila hoʻopaʻa ʻia i ka hoʻonui mua ʻia e Klenow fragment exo- (Kf exo-), calf thymus DNA polymerase alpha (pol alpha). ) a i ʻole DNA polymerase beta kanaka (pol beta).E like me nā hōʻike mua, ua hoʻokomo ʻia ka dAMP a me ka dCMP ma ke alo o 8-oxoG me nā polymerases DNA ʻekolu.ʻO ka mea e mahalo ai, ua ʻike ʻia ka oxidation o 8-oxoG i mea e hoʻokomo ai i ka dAMP a me ka dGMP e kū pono ana i ka liona e Kf exo- me ka pale ʻana o ka hoʻonui mua ʻana i ke kahua o ke kumu hoʻololi.Eia kekahi, he poloka ka lesion i ka wā o ka synthesis DNA e pol alpha a me pol beta.Hōʻike nā hoʻokolohua me kahi oligonucleotide template 8-oxoA i hoʻololi ʻia ʻo 8-oxoA a me kahi ʻano oxidized o 8-oxoA hoʻokomo pololei o dTMP e Kf exo-.ʻO ka loiloi spectrometric nui o nā oligonucleotides i loko o 8-oxoG ma mua a ma hope o ka hoʻokahe ʻia ʻana me IrCl62-kūlike me ka oxidation o ka pūnaewele 8-oxoG, ka hopena o ka hoʻokumu ʻana i kahi guanidinohydantoin moiety e like me ka huahana nui.ʻAʻole i loaʻa nā hōʻike no ka hoʻokumu ʻana i nā wahi abasic.Hōʻike kēia mau hualoaʻa i kahi ʻano oxidized o 8-oxoG, he guanidinohydantoin paha, hiki ke kuhikuhi i ka heluhelu hewa a me ka hoʻokomo hewa ʻana o nā dNTP i ka wā o ka synthesis DNA.Inā i hana ʻia ia hana i loko o ka vivo, e hōʻike ana ia i kahi lesion mutagenic kiko e alakaʻi ana i nā hoʻololi G--> T a me G--> C.Eia naʻe, hōʻike mua ke ʻano oxidized o 8-oxoA i ka hoʻokomo pololei ʻana o T i ka wā DNA synthesis me Kf exo-.
