Nā huahana

ʻO APS-5 kahi substrate chemiluminescent e pili ana i ka 9,10-dihydroacridine, i hoʻohana nui ʻia no ka ʻike ELISA o nā pūhui me nā conjugates AP phosphatase alkaline;9,10-dihydroacridine i loko o ka hale o APS-5 Hiki i ka hydroacridine hale ke hoʻokuʻu i ka malamalama mau, kūpaʻa a me ka maikaʻi ma lalo o ka hana a AP, a he kūpono loa ʻo APS-5 no ka phosphatase activity solution assay and phosphatase enzyme-linked immunoassay.

Ma ke ʻano he luminescent probe, hoʻohana ʻia ia i ke aʻo ʻana i nā ʻāpana gene.Hoʻopili ʻia ka hopena me ka acridan (9,10-dihydroacridine) ma ke ʻano he substrate a me ka alkaline phosphatase, e hana ana i ka chemiluminescence kiʻekiʻe.Hāʻawi i ka naʻau maikaʻi a me ka maʻalahi o ka hoʻohana ʻana no ka conjugation alkaline phosphatase i ka wā ʻike chemiluminescent.ʻIke ʻia ka alkaline phosphatase ma lalo o 10-19 mol, piʻi wikiwiki e hōʻemi i ka manawa ʻike a hoʻonui i ka throughput, a ua hoʻolālā ʻia ka slope o ka linear calibration curve me ka logarithmic like me 1.0.Hoʻokahi nui a ʻoi aʻe paha o ka enzyme e hoʻopuka i hoʻokahi nui a ʻoi aʻe paha o ka luminescence, hoʻomau mau ka luminescence-ʻaʻole koi nui i ka manawa hoʻāʻo.Hiki ke heluhelu ʻia ka ikaika o ka luminescence mai ka ʻōkuhi linear calibration i hana ʻia i kēlā me kēia manawa, a ʻaʻole i ʻike nā hopena analytical i ka mahana ma waena o 22 ° C - 35 ° C, e hōʻemi ana i ka pololei e pono ai ke kāohi i ka mahana.

pono:

1 ʻIke kiʻekiʻe - hiki ke ʻike ʻia nā māka protein ma lalo o 5pg;

2 Kiʻekiʻe luminous ikaika - ka luminous peak hiki ke hiki i ka pōkole manawa;

3 Hoʻomau a paʻa ka luminescence - ʻaʻole pili kona kaʻina luminescence e ka mahana i loko o 25-35 ℃, a ʻaʻole koi i ka mālama wela.

Hoʻohālikelike noiʻi o nā substrate luminescent maʻamau i hoʻohana ʻia APS-5 (193884-53-6) a me AMPPD (122341-56-4)

ʻO APS-5 (193884-53-6) a me AMPPD (122341-56-4) he mau substrate chemiluminescent no ka alkaline phosphatase, a ua kapa ʻia lāua ʻelua he ultra-high sensitivity detection reagents, no laila aia kēia mau reagents ʻelua i ka ʻike. ʻo wai ka mea ʻino?Pehea e koho ai i ka wā e hoʻohana ai?

Ma ka lima hoʻokahi, ke hoʻohana ʻia ʻo APS-5 e like me kahi substrate detection alkaline phosphatase, hiki i ka ʻike ʻike ke hiki i ka 10-19 molar concentration, hiki i ka manawa hoʻomohala kala ke hiki i ka piko kiʻekiʻe ma kahi o 10 kekona, a mau ka fluorescence no kahi lōʻihi. manawa.Ma kēia mau mea, hiki ke ʻōlelo ʻia he waiwai maikaʻi loa ka APS 5.Eia nō naʻe, ua kaupalena ʻia ka APS-5 i ka wela o ka noi, a ʻoi aku ka maikaʻi o ka wela o ka waihoʻoluʻu ma lalo o 20'C.Ma kekahiʻaoʻao, i ka wā e hoʻohanaʻia ai ka AMPPD ma keʻano he substrate detection no ka alkaline phosphatase, ua kapaʻia kaʻikeʻikeʻike he ultra-high sensitivity, akā,ʻaʻole hiki ke hiki i ke kiʻekiʻe o APS 5. ʻO ka manawa hoʻolālā kala e hiki i ka piko kiʻekiʻe ma kahi o 30 kekona. , a ʻoi aku ka lōʻihi o ka fluorescence.He maikaʻi loa ka AMPPD i nā waiwai i luna, akā ʻoi aku ka haʻahaʻa i ka APS5, akā ʻaʻole koʻikoʻi kona ʻano kala e like me APS5, a ʻoi aku ka maʻalahi o ka mālama ʻana o 2--8C ma mua o APS-5.I ka hōʻuluʻulu manaʻo, ʻoiai ua ʻoi aku ka APS-5 ma mua o AMPPD i ka ʻike pololei ʻana, e nalowale ana i ka paʻa.Pehea e koho ai ma muli o nā pono kikoʻī o ka ʻike.

1. Introduction of APS-5 (193884-53-6):

inoa Pelekania: APS-5

Helu CAS: 193884-53-6

Huina mole: C21H15ClNO4PS Na2

Kaumaha molekula: 489.819

Ke ʻano: pauka paʻa keʻokeʻo keʻokeʻo

Maʻiʻo: ≥98%

Nā kikoʻī: 1g, 5g, 10g

Nā kūlana mālama: -20 ℃ mālama

(4-Chlorophenylmercapto)(10-methyl-9,10-dihydroacridine methylene) phosphate disodium paʻakai, i kapa ʻia ʻo APS-5, he reagent chemiluminescent a hoʻohana ʻia ma ke ʻano he substrate chemiluminescent.Hāʻawi i ka naʻau maikaʻi a me ka maʻalahi o ka hoʻohana ʻana no ka conjugation alkaline phosphatase i ka wā ʻike chemiluminescent.Hoʻopili ʻia ka hopena me ka acridan (9,10-dihydroacridine) ma ke ʻano he substrate a me ka alkaline phosphatase, e hana ana i ka chemiluminescence kiʻekiʻe.ʻO APS-5 kahi reagent kūpono no ka hoʻoholo ʻana i ka hana phosphatase ma o ka hoʻonā ʻana a me nā immunoassays pili i ka enzyme phosphatase.Ma ke ʻano he luminescent substrate, loaʻa iā APS-5 ka ʻike kiʻekiʻe a me ka manawa pane pōkole.

2. Hoʻopuka kikoʻī o AMPPD (122341-56-4)

inoa Pelekane: AMPPD

Helu CAS: 122341-56-4

Huina mole: C18H23O7P

Kaumaha molekula: 382.344781

Nānā: ka pauka keʻokeʻo

Maʻemaʻe: ≥97%

Nā kikoʻī: 1g, 5g, 10g

Nā kūlana mālama: -20 ℃ mālama

E mālama: Pono e maloʻo e pale i ka hoʻoheheʻe pinepine ʻana a me ka lyophilization