Nā huahana

1. He mea koʻikoʻi ka hoʻololi ʻana o ka hana o nā enzymes e pili ana me ka metabolism carbohydrate no ka ʻuala i hoʻokomo ʻia i ke anuanu (CIS).Ua hoʻopaʻa ʻia kahi moʻo RING finger gene SbRFP1 a ua ʻike ʻia kona hōʻike ʻana he hiki ke anu i loko o nā ʻuala ʻuala o nā genotypes-kūpaʻa CIS.ʻO ka hoʻololi ʻana o SbRFP1 i loko o ka ʻuala i hōʻoia i kāna kuleana i ke kāohi ʻana i ka hana β-amylase a me ka invertase, a no laila ua lohi i ka hōʻemi ʻana o ka starch a me ka sucrose a me ka hōʻiliʻili ʻana o ka hoʻohaʻahaʻa ʻana i nā kō i loko o nā kōpaʻa mālama anuanu.Manaʻo ikaika kēia mau ʻike e hiki ke hana ʻo SbRFP1 ma ke ʻano he mea hoʻoponopono maikaʻi ʻole o BAM1 a me StvacINV1 e hoʻohaʻahaʻa i ka hōʻiliʻili ʻana o ka hoʻemi ʻana i nā kō i ke kaʻina o ka ʻuala CIS.

2. ʻO ka pahuhopu o kēia hana ʻo ia ke aʻo ʻana i ka mana o ka kuke ʻana i ka mahu ma luna o ka pectin methylesterase (PME) a ​​me nā hana endogenous α- a me β-amylase i loko o nā ʻiʻo like ʻole (cortex a me pith) o ka ʻuala maka a me ka wela i mālama ʻia cv.ʻAgeria.Ua koho ʻia ʻekolu mau mahana kuke like ʻole (55, 70 a me 85 °C).No kēlā me kēia hoʻāʻo kuke ʻana, ua hoʻopaʻa ʻia nā ʻaoʻao wela o ka manawa a ua hōʻike ʻia ke degere o ka kuke ʻana ma ke ʻano o ka mea kuke. , me ka nui loa o ka cortex (0.3745 ± 0.0007 µmol galacturonic acid (GA) g(-1) hou kaumaha (FW) min(-1) ) ke hoohalikeia me ka pith (0.2617 ± 0.0012 µmol GA g(-1) FW min(-1) ).Ua manaʻo pū ʻia ka loaʻa ʻana o nā isoforms wela wela a paʻa i ka PME i loko o nā ʻuala i manaʻo ʻia.ʻO ka hoʻomaʻamaʻa ʻana i ka wela e ka mahu ua hoʻemi nui ʻia nā hana endogenous α- a me β-amylase i loko o nā ʻiʻo ʻelua i hoʻohālikelike ʻia me ka pota maka i, ʻoiai ʻaʻole hoʻopau piha.Ua ʻike ʻia ka māhele ʻokoʻa ʻana o nā enzymes hoʻohaʻahaʻa i ka starch i loko o ka ʻuala maka.ʻAgeria.Pono nā noiʻi hou e hōʻoia i nā hopena i loaʻa.