Nā huahana

ʻO Bicine he zwitterionic amino acid buffer, e hana ana ma ka pae pH 7.6-9.0 (pKa o 8.26 ma 25°C).Paipai ʻia no ka hana biochemical wela haʻahaʻa.Hoʻohana ʻia ʻo Bicine no ka hoʻomākaukau ʻana i ka hopena substrate paʻa no ka hoʻoholo ʻana i ka serum guanase.Ua paʻi ʻia ka hoʻohana ʻana i ka bicine ma kahi ʻano chromatography hoʻololi ion exchange no ka hoʻonā protein.Ua hoʻohana ʻia ʻo Bicine i ka peptide a me ka crystallization protein.He haʻawina kinetic o kahi quaternary transition-state analogue complex o ka creatine kinase i hoʻohana ʻia i ka bicine i loko o ka pale pane.Ua wehewehe ʻia kahi ʻōnaehana pale multiphasic no SDS-PAGE o nā protein a me nā peptides i hoʻokomo i ka bicine.

ʻO nā pā alveolar hoʻokahi i hoʻopaʻa ʻia i nā haʻawina hoʻopaʻapaʻa lōʻihi i ka paʻakai a me ka Bicine (0.2 M) e hele ana i kahi palaho i ka ʻili o ke kino (TTD).Ua nānā mākou i ka hopena o nā hoʻonā like ʻole ma kēia TTD a ʻimi i nā loli kūpono i ka ultrastructure.Ua ʻoki ʻia ka parenchyma māmā i nā paia alveolar hoʻokahi (30 X 30 X 150 microns) i loko o ka paʻakai phosphate-buffered (0.15 M).Hoʻololi ʻia i kahi ʻauʻau ʻauʻau lōʻihi, ua hoʻokomo ʻia ka ʻiʻo i loko o ka Bicine, paʻakai, paʻa Hank's solution, 0.25% Alcian blue in saline, a i ʻole ka sodium dodecyl sulfate solution, no nā manawa loli.Hoʻopili ʻia ma kahi hoʻonui i hāʻawi ʻia me ka ikaika kiʻekiʻe i ana ʻia i ka manawa, ua hoʻopaʻa ʻia kēia mau kiko like i ka buffered glutaraldehyde/tannic acid a ua hana ʻia no ka microscopy electron.ʻO nā paia alveolar hoʻokahi i hoʻokomo ʻia i ka paʻakai a i ʻole Bicine i hōʻike i kahi TTD holomua.Ua ʻike ʻia nā vacuoles a i ʻole nā ​​​​wāwae i loko o ka interstitium me ka disorganization cellular i holomua me ka TTD.ʻIke ʻia i loko o 0.3 h, ua holomua maikaʻi nā loli ma 0.6 h.I loko o ka sodium dodecyl sulfate (70 mM), akā naʻe, ʻaʻohe TTD a ma ke ʻano ʻaʻohe interstitium, me nā membrane basement a me nā protein fibrous i koe.I loko o ka hoʻonā Hank paʻa a i ʻole 0.25% Alcian blue ua mālama maikaʻi ʻia ka matrix interstitial, cell morphology a me ka ʻāʻī ʻana no ka hola 1.Hōʻike kēia haʻawina e hana ʻia ka leaching o ka matrix interstitial i ka paʻakai a i ʻole Bicine, a he mea nui ka matrix paʻa no ka mālama ʻana i ka ʻāʻī.