Nā huahana

Nā ʻakika zwitterionic butanesulfonic hou e hoʻolōʻihi i ka pae alkaline o nā ʻohana ʻehā o nā mea pale maikaʻi: loiloi no ka hoʻohana ʻana i nā ʻōnaehana olaola.

ʻEhā mau mea hoʻopaʻa wai zwitterionic butanesulfonic hou e pili ana i nā ʻohana ʻehā o nā buffers maikaʻi i loiloi ʻia no ka hoʻohana ʻana i nā ʻōnaehana olaola.ʻO kēia mau mea pale, me nā waiwai pKa mai 7.6 a i 10.7, ua hoʻohālikelike ʻia me nā ʻano pale ʻē aʻe mai ka ʻohana hoʻokahi a me nā pale pili ʻole e hoʻoholo ai i ko lākou hopena i ka hana enzyme a me ka ulu ʻana o ka microbial.Ua hoʻāʻo ʻia ka hana o nā enzyme ʻehā me nā waiwai pH maikaʻi loa i ka pae alkaline: beta-galactosidase, esterase, phosphodiesterase a me alkaline phosphatase.Ma keʻano laulā, ua hāʻawi ka hana maikaʻi i nā mea pale maikaʻi a pau, e komo pū ana me nā mea hoʻoheheʻe waika butanesulfonic hou;akā naʻe, ua ʻokoʻa ka hana o kekahi mau enzyme me kekahi mau pale.Hōʻike maʻamau ka hoʻololi ʻana o ka hana hoʻohālikelike ʻia me ka ʻohana o Good buffers.ʻO ka beta-Galactosidase, ka mea nui, ua hōʻike i ka hana ʻoi aku ka nui me nā pale maikaʻi ma mua o ka phosphate a i ʻole Tris buffers.Pēlā nō, ua kūlike ka ulu ʻana o ʻehiku mau maʻi bacterial, me nā ʻokoʻa liʻiliʻi, no ka ʻohana maikaʻi a pau o nā buffers me Tris ke kāohi pinepine nei i ka ulu.ʻO ka helu ʻana o ka alkaline phosphatase i hoʻohui ʻia i nā antibodies he mea koʻikoʻi i nā noi he nui i ka biology molecular.Ua hāʻawi kekahi mau mea pale maikaʻi i nā hōʻailona maikaʻi ke hoʻohālikelike ʻia me Tris ma ka pH 9.5 no ka ʻike ʻana i nā protein me ka hoʻohana ʻana i nā antibodies alkaline phosphatase-conjugated.