Nā huahana

Hoʻohana ʻia no ka hoʻokumu ʻana i CAPS buffer, kahi zwitterionic buffer pono i ka laulā o ka pH 7.9-11.1.Hoʻohana nui ʻia ʻo CAPS buffer i nā hoʻokolohua Western a immunoblotting a me ka hoʻopili ʻana i ka protein a me ka ʻike.Hoʻohana ʻia i ka hoʻololi electro o nā protein i PVDF (sc-3723) a i ʻole nā ​​membrane nitrocellulose (sc-3718, sc-3724).ʻO ka pH kiʻekiʻe o kēia paʻa e pono ai no ka hoʻololi ʻana o nā protein me kahi pI> 8.5.a me ka liʻiliʻi liʻiliʻi me nā enzymes a i ʻole nā ​​protein, nā hopena paʻakai liʻiliʻi.

I loko o ka capillary zone electrophoresis e emi ana ka wikiwiki electrophoretic o kahi ion i ka hoʻonui ʻia ʻana o ka ʻike o ka hopena electrolyte hope.Hoʻololi ʻia kēia ma muli o ka hoʻololi ʻana i ka neʻe electrophoretic o ka ion (muep) a me ka hoʻololi ʻana i ka ikaika ʻupena e pili ana iā ia, ʻo ia hoʻi ka ikaika o ke kahua uila (Eeff).Hoʻololi ʻia ka neʻe electrophoretic o kahi ion ma o ka hoʻololi ʻana i ka viscosity piha o ka solution electrolyte a me nā loli i ka nui o ka ion.Hoʻololi nui ʻia ʻo Eeff e nā loli i ka nui o ka hopena asymmetry charge a me ka hopena electrophoretic, ʻo ia mau mea ʻelua e hoʻopaneʻe i ka neʻe o nā ion.I loko o kēia haʻawina, ua hoʻohana ʻia ka ʻenehana hōʻailona ʻekolu e aʻo i ka hopena o ka ʻike electrolyte hope (0.02-0.08M 3- [cyclohexylamino] -1-propanesulfonic acid a me ka ion counter (Li, Na, K, a me Cs) ma Eeff. Ua ʻike ʻia ʻo ka ʻike ʻana o ka electrolyte hope e pili pono iā Eeff a ua hoʻokokoke ʻo Eeff i E i ka wā e hoʻokokoke ai ka ʻike o ka electrolyte hope i ka zero. , Ua emi iho ka Eeff. Ua hooiaio pono ka hana hoailona ekolu no ia mau manao hooholo.