Nā huahana

Hōʻike ʻia kahi kaʻina hana hoʻopaʻa no ka hoʻohana ʻana me nā ʻāpana kikoo glycol methacrylate i hoʻopaʻa ʻole ʻia i ka hoʻokomo ʻana i ka plastic a wehe ʻole i nā ʻāpana mai nā kiʻi aniani.ʻO ka mea pena kumu he celestine blue B. Ua hoʻomākaukau ʻia ma ka mālama ʻana i 1 g o ka wai me ka 0.5 ml sulfuric acid concentrated.A laila e hoʻoheheʻe ʻia me ka hoʻonā.E hoʻohui i ka 14 ml glycerine i 100 ml 2.5% ferric ammonium sulfate a hoʻomehana i ka hopena i 50 C. Ma hope e hoʻoponopono i ka pH i 0.8 a i 0.9 ʻO ka wai hoʻoheheʻe waika he 0.075% ponceau de xylidine a me 0.025% acid fuchsin i 10% acetic acid.Hoʻokomo ʻia nā paheʻe i loko o nā ʻāpana plastik maloʻo i loko o ka solution blue celestine no ʻelima mau minuke a i loko o ka solution ponceau-fuchsin no ʻumi mau minuke me kahi holoi wai.Ma hope o ka holoi hope ʻana, hoʻomaloʻo ʻia nā ʻāpana a uhi ʻia.ʻO kēia kaʻina hana hoʻopaʻapaʻa e kala i nā ʻiʻo e like me nā kaʻina hematoxylin a me eosin.