Nā huahana

ʻO ka brucellae nā mea etiological o brucellosis, kahi zoonosis i hoʻolaha ʻia ma ka honua holoʻokoʻa.ʻO kēia mau maʻi bacteria he facultative intracellular parasites a no laila hiki iā lākou ke hoʻoponopono i kā lākou metabolism i nā kaiapuni keu a me ka intracellular i ʻike ʻia i ka wā o kahi pōʻai infectious.Eia naʻe, ʻaʻole maopopo i kēia ʻano o ka biology Brucella, a ʻaʻole ʻike ʻia nā meaʻai i loaʻa i loko o ka niche intracellular.Maʻaneʻi, ua noiʻi mākou i nā ala waena o C metabolism i hoʻohana ʻia e Brucella abortus ma ka holoi ʻana i ka fructose-1,6-bisphosphatase putative (fbp a me glpX), phosphoenolpyruvate carboxykinase (pckA), pyruvate phosphate dikinase (ppdK), a me ka enzyme malic (mae) genes.I loko o ka gluconeogenic akā ʻaʻole i loko o ka media waiwai, ua pilikia nui ka ulu ʻana o ka ΔppdK a me ka Δmae mutants a ua hoʻemi ʻia ka ulu ʻana o ka mutant Δfbp-ΔglpX pālua.I loko o nā macrophages, ʻo ka ΔppdK a me ka Δmae mutants wale nō i hōʻike i ka hoʻonui ʻia ʻana, a ua hōʻoia ʻia nā haʻawina me ka mutant ΔppdK ua hiki i ka niche replicative.Pēlā nō, ua hoʻopau ʻia nā mutants ΔppdK a me Δmae i nā ʻiole, ua hoʻomaʻemaʻe ʻia ka mea mua ma ka pule 10 a ʻo ka hope e hoʻomau nei ma mua o 12 mau pule.Ua noiʻi pū mākou i ka pōʻaiapili glyoxylate.ʻOiai ua hoʻonui ʻia ka hana hoʻolaha aceA (isocitrate lyase) ma ke ʻano waiwai, ʻaʻohe hopena o ka hoʻopau ʻana i ka aceA in vitro a i ʻole ka hoʻonui ʻana i nā macrophages a i ʻole spleens ʻiole.Hōʻike nā hopena i ka ulu ʻana o B. abortus intracellularly me ka hoʻohana ʻana i ka hāʻawi palena ʻia o nā sugars 6-C (a me 5-C) i uku ʻia e ka glutamate a me nā waikawa amino ʻē aʻe e komo ana i ka pōʻai Krebs me ka ʻole o ke kuleana koʻikoʻi o ka glyoxylate shunt.