Nā huahana

ʻIke pinepine ʻia ka adsorption o nā protein i nā pilina paʻa-wai e hoʻoikaika i ka hoʻokumu ʻana o nā ʻāpana hiki ke hoʻoheheʻe ʻia a me nā ʻāpana nui, subvisible, a ʻike ʻia, akā paʻakikī ke nānā pono i nā pae koʻikoʻi o kēia kaʻina hana.ʻO ka hui ʻana i hoʻopili ʻia e ka adsorption i ka wai-silicon oxide (SiOx) interfaces, e like me ka hydrated glass surfaces, i ʻike ʻia he hana o ka pH a me ka ikaika ionic no alpha-chymotrypsinogen (aCgn) a no kahi antibody monoclonal (IgG1).Ua ʻae ʻia ka hoʻomaʻemaʻe ʻana i nā ʻili o SiOx ma hope o ka hoʻomaʻemaʻe ʻana i ka neutron no nā ʻāpana protein, a ma hope o nā ʻanuʻu "holoi" ʻē aʻe.Hōʻike ʻia nā mea hōʻuluʻulu i ka hopena ma hope o ka "holoi ʻana" i ka ʻili e ka neutron scattering, microscopy, a me fluorescence spectroscopy.ʻO nā molekala IgG1 i hoʻonohonoho mua ʻia i ka "flat" e kūʻē i ka ʻili SiOx, me ka hoʻoneʻe ʻana o ka papa protein mua i kahi liʻiliʻi loa, ʻoiai ua holoi maʻalahi ʻia kahi overlayer diffuse.Ua kūpaʻa nā molekala aCgn i ka desorption i ka wā i ʻike ʻia ai e wehe ʻia ma ke kikowaena, akā ua maʻalahi ka wehe ʻana.No nā hihia i hoʻopaʻa ikaika ʻia, ʻo ka protein i hoʻopau ʻia he hui ʻana o ka monomer a me nā mea liʻiliʻi o nā HMW aggregates (no aCgn) a i ʻole nā ​​​​particle subvisible (no IgG1).ʻO nā hoʻololi i ka adsorption a / a i ʻole ka wehe ʻana me ka pH i hōʻike ʻia he mea nui ka pilina electrostatic i nā hihia āpau.