Nā huahana

ʻO CTP: phosphocholine cytidylyltransferase α (CCTα) he enzyme nuklea e hoʻopaʻa i ka pae palena palena ma ke ala CDP-choline no ka phosphatidylcholine (PC) synthesis.ʻO ka hoʻoulu ʻana o ka lipid o CCTα ka hopena i kona unuhi ʻana i ka envelop nuklea a me ka hoʻonui ʻana o kahi ʻupena membrane intranuclear i kapa ʻia ʻo ka nucleoplasmic reticulum (NR) e kahi mīkini e pili ana i ka deformation membrane.Pono ʻia nā lamina nuklea no ka paʻa a me ka hoʻonui ʻana o ka NR, akā inā ʻaʻole ʻike ʻia kēia ʻano ʻokoʻa, a i ʻole ka lamina nuklea ma ke ʻano nui, no ka PC synthesis.No ka nānā ʻana i kēia pilina, ua hoʻopau ʻia ka lamina nuklea e RNAi a i hoʻopau ʻia e ka hōʻike ʻana o ka Hutchinson-Gilford progeria syndrome (HGPS) mutant lamin A (progerin), a ua kālailai ʻia ka hopena ma CCTα a me choline metabolism.ʻO ka siRNA-mediated silencing o ka lamin A/C a i ʻole lamin B1 i loko o nā keena CHO e hōʻemi i ka NR ʻaʻohe hopena i ka synthesis PC, ʻoiai ʻo ke kīkē pālua ʻaʻole i hoʻokaʻawale i ke ala.E hōʻoia ana i kāna kuleana liʻiliʻi i ka synthesis PC, ʻo 10% wale nō o ka transiently overexpressed choline / ethanolamine phosphotransferase i ʻike ʻia i ka NR.I loko o nā pūnaewele CHO, CCTα he nucleoplasmic a hui pū me ka GFP-progerin i loko o nā pā nuklea a me nā invaginations;akā naʻe, ua hōʻike nā fibroblasts HGPS i ka hāʻawi like ʻole o CCTα i loko o ka cytoplasm a me ka envelope nuklea i hui pū ʻia me ka hōʻemi 2-fold i ka synthesis PC.I loko naʻe o kona hoʻololi ʻia ʻana, ua hōʻike ʻia nā hoʻokolohua choline-labelling ʻaʻole i hoʻopilikia ʻia ka hana CCT, a ua ʻike ʻia ka inhibition o PC synthesis i hoʻemi ʻia ka hana o kahi mea lawe choline sensitive hemicolinium.Manaʻo mākou e hui like ʻo CCTα a me nā lamins e hana i ka NR, akā ʻo ke ʻano holoʻokoʻa o ka envelope nuklea he hopena liʻiliʻi i ka hana CCT a me ka synthesis PC.