Nā huahana

Hoʻopili ka Tet 5-methylcytosine dioxygenases i ka demethylation DNA ma o ka hana ʻana i ka 5-hydroxymethylcytosine a me nā huahana oxidized hou aʻe.Hōʻike nui ʻia ʻo Tet1 a me Tet2 i loko o nā cell pluripotent mouse a hoʻohaʻahaʻa ʻia i nā ʻokoʻa like ʻole i loko o nā cell somatic, akā ʻaʻole maopopo nā hana transcriptional.Maanei mākou i wehewehe ai i ka mea hoʻolaha a hoʻonui i nā kāʻei kapu ma Tet1 a me Tet2.Ma loko o kahi 15-kb "superenhancer" o Tet1, aia ʻelua mau kahua hoʻomaka transcription (TSS) me nā ʻano hoʻāla ʻokoʻa i ka wā o ka hoʻomohala ʻana.ʻO kahi 6-kb mea hoʻolaha ʻāina ma luna o ka TSS mamao e ikaika loa i nā cell pluripotent naive, hōʻike autonomously Tet1 hōʻike i loko o kahi ʻōnaehana transgenic, a hele wikiwiki i ka DNA methylation a me ka silencing ma ka hoʻokaʻawale ʻana i nā cell moʻomeheu a me ka epiblast maoli.Hoʻohana ʻia ka lua o ka TSS i lalo, pili me kahi mea hoʻolaha waiwai CpG i nāwaliwali, e ka mea hoʻonui pili i nā pūnaewele embryonic stem naive (ESCs) a me nā cell epiblast-like primed (EpiLCs).Loaʻa iā Tet2 kahi mokupuni CpG p romoter me ka pluripotency-kūʻokoʻa hana a me kahi ESC-specific distal intragenic enhancer;ua hoʻohaʻahaʻa koke ʻia ka mea hope i nā EpiLC.Hōʻike kā mākou haʻawina i nā ʻano ʻano hoʻoponopono transcriptional ma Tet1 a me Tet2 i ka wā o ka neʻe ʻana o ka mokuʻāina o ka hoʻomohala mua.Hiki i nā mea hoʻolaha transgenic hou me Tet1 a me Tet2 cis-regulatory domain ke hoʻokaʻawale i nā loli nuanced i nā mokuʻāina pluripotent a me nā ʻano like ʻole epigenetic.