dCTP, 2'-Deoxycytidine-5'-triphosphoric acid disodium paʻakai Cas:102783-51-7 Pada Keʻokeʻo
| Helu Helu | XD90584 |
| inoa mea kūʻai | dCTP, 2'-Deoxycytidine-5'-triphosphoric acid disodium paʻakai |
| CAS | 951-78-0 |
| ʻĀpana Molekala | C9H12N2O5 |
| Kaumaha Molecular | 228.20 |
| Nā kikoʻī mālama | -20 °C |
| Kānāwai Kūʻai Kūlike | 29349990 |
Huahana Huahana
| Ka nana aku | Pauda keʻokeʻo |
| Hoʻāʻo | 99% |
Loaʻa i nā pūnaewele Eukaryotic kahi koena maikaʻi o nā minuke o nā deoxyribonucleoside triphosphates ʻehā (dNTPs), lawa wale no kekahi mau minuke o ka hana hou ʻana o DNA.ʻO ka hemahema a me ke koena o ka dNTP hoʻokahi hiki ke hopena i ka piʻi ʻana o ka hoʻololi ʻana, ka hoʻoponopono DNA hewa a i ʻole ka pau ʻana o ka DNA mitochondrial.Hoʻohālikelike ʻia nā dNTP e kahi hoʻāʻo enzymatic kahi i hoʻohui ʻia ai ka radioactive dATP (a i ʻole radioactive dTTP i ka assay no dATP) i loko o nā oligonucleotides synthetic kikoʻī e kahi DNA polymerase i kūlike me ka ʻike o ka dNTP ʻike ʻole.Hiki iā mākou ke hoʻololi i ka ribonucleotide like ʻole no ka dNTP i ʻike ʻole ʻia e alakaʻi ana i kekahi mau manawa i kahi overestimation nui o nā dNTP.Ke wehewehe nei mākou i nā kūlana hoʻokolohua no kēlā me kēia dNTP e pale i ka hoʻohui ʻana o ribonucleotide.No ka ho'āʻo dTTP a me dATP ua lawa ia e hōʻemi i ka hoʻohaʻahaʻa ʻana o ka enzyme Klenow a me ka dATP i kapa ʻia (a i ʻole dTTP);no ka dCTP a me ka dGTP pono mākou e pani i ka enzyme Klenow me ka Taq DNA polymerase a i ʻole Thermo Sequenase.Manaʻo mākou i loko o kekahi mau hōʻike mua i hoʻohui ʻia ai ka ribonucleotide i kumu nui loa no ka dGTP a me ka dCTP.
