Nā huahana

Hoʻonui ʻia ke koʻikoʻi oxidative ma lalo o nā kūlana hyperglycemic, ma o ka launa pū ʻana o nā AGE me nā mea loaʻa RAGE a ma o ka hoʻāla ʻana o ka interleukin mediated transcription signalling, ua hōʻike ʻia i ka maʻi kanesa.Ke ʻimi ʻia nei nā hoʻololi protein no kā lākou kuleana i ka hoʻomohala ʻana a me ka holomua ʻana o ka maʻi maʻi a me ka pane autoantibody e kūʻē iā lākou e loaʻa ana ka hoihoi ma ke ʻano he noiʻi no ka ʻike mua ʻana i ka maʻi.Ua nānā kēia haʻawina i nā loli o ka histone H1 ma ka hoʻololi ʻana e ka methylglyoxal (MG) a me kāna mau hopena i ka auto-immunopathogenesis o ka maʻi kanesa.Hōʻike ka histone i hoʻololi ʻia i nā koena ʻala, hoʻololi i ka tyrosine microenvironment, intermolecular cross linking and generation of AGEs.Ua hōʻike ʻia ka masking o nā ʻāpana hydrophobic a me kahi hoʻololi hypsochromic i ka fluorescence kikoʻī o ANS.ʻO MG i hoʻopaʻa ʻia i ka histone H1 e alakaʻi ana i ka hōʻiliʻili ʻana o nā carbonyl reactive.ʻO nā ana CD UV mamao loa i hōʻike ʻia i ka hoʻonui ʻia ʻana o ka di-carbonyl i hoʻonui ʻia i ke ʻano alpha a me ka hoʻokomo ʻana o ka hoʻololi ʻana o ka pepa beta;a me nā haʻawina thermal denaturation (Tm) i hōʻoia i ke kūpaʻa wela o ka histone i hoʻololi ʻia.Hōʻike ka hōʻike FTIR i ka amide I band shift, ka hana ʻana o kahi hui carboxyethyl a me nā haʻalulu N-Cα i ka histone i hoʻololi ʻia.Ua hōʻoia ka loiloi LCMS i ka hoʻokumu ʻana o Nε-(carboxyethyl)lysine a me nā haʻawina microscopic electron i hōʻike i ka hoʻokumu ʻana o ka amorphous aggregate.Ua hōʻike ka histone i hoʻololi ʻia i ka hoʻopaʻa ʻana me ka DNA.ʻO ka H1 i hoʻololi ʻia i hoʻokomo ʻia i nā antibodies kiʻekiʻe kiʻekiʻe i loko o nā lapeti a ʻo ka IgG i hoʻokaʻawale ʻia i ke ʻano sera o nā rapeti i hoʻopaʻa ʻia me ka H1 i hoʻololi ʻia i hōʻike ʻia i ka paʻa kikoʻī me kāna immunogen i ka nānā ʻana o Western Blot.Ua hoʻokaʻawale ʻia ʻo IgG mai ka sera o nā mea maʻi me ka maʻi kanesa prostate, ka maʻi kanesa o ka umauma a me ke kanesa o ke poʻo a me ka ʻāʻī ʻāina i hōʻike ʻoi aku ka maikaʻi o ka ʻike no nā neo-epitopes ma ka histone i hoʻololi ʻia, e hōʻike ana i ka hele ʻana o nā autoantibodies i ka maʻi kanesa.No ka mea e hōʻike ana nā hōʻike i kahi loulou ma waena o AGE-RAGE axis a me carcinogenesis, ʻo ka glycoxidation o ka histone H1 a me kāna immunogenicity e hoʻomākaukau i nā ala no ka hoʻomaopopo ʻana i ke kuleana o nā protein nuklea i hōʻino ʻia i ka glycoxidatively i ka maʻi kanesa.