Nā huahana

1. He mau mea hana koʻikoʻi nā ʻano hana hoʻohālikelike metabolite no ke ana ʻana i nā loko metabolite i nā ʻōnaehana olaola.ʻOiai ʻo ka hapa nui o nā ʻano metabolite profiling e hōʻike ana i ka ikaika pili a hilinaʻi paha i kekahi mau kūlana kūloko e hōʻike ana i nā metabolites āpau, ʻo ke koi hope loa no ka wehewehe nui ʻana o ka wai metabolite i loko o nā cell biological a me nā wai ʻo ia ka hoʻoholo paʻa ʻana.Ke hōʻike nei mākou ma ʻaneʻi i kahi ʻano hana hoʻohālikelike metabolite i hoʻopaʻa ʻia i ka chromatography/tandem mass spectrometry (GC/MS/MS) e hiki ai ke helu pono i nā metabolites āpau i ʻike ʻia.Hoʻokumu ʻia ke ala ma ka methyl chloroformate derivatization a me ka helu ʻana ma o ka spiking samples me nā kūlana metabolite i hoʻokaʻawale ʻia me nā reagents deuterated derivatization.Hoʻololi ʻia ka ionization hopena electron maʻamau me ka ionization kemika maikaʻi ʻoiai ʻo ka mea hope i kahi ʻoi aku ka nui o ka mālama ʻana i ka ion molecular a me nā ʻāpana kaumaha molekala kiʻekiʻe.Ua maʻalahi kēia i ke koho ʻana i nā hoʻololi MS/MS kūʻokoʻa i waena o nā metabolites coeluting.I kēia manawa, ʻo ka moʻolelo GC/MS/MS me 67 mau metabolites maʻamau o ka hapa nui o nā pūʻulu o nā amino a me nā waikona noamino.Hōʻike mākou i ka hoʻohana ʻana o ke ʻano ma ka mimi a me nā laʻana serum.He hoʻomaikaʻi koʻikoʻi ke ʻano o ke kaʻina hana i kēia manawa no ka quantitative GC/MS metabolite profiling o nā waikawa amino a me nā ʻakika organik nonamino.

ʻO 2.24S-Hydroxycholesterol (24OHC) a me 27-hydroxycholesterol (27OHC) ʻelua mau oxysterols like ʻole o nā kumu like ʻole--ʻo ka mea mua i hoʻokumu wale ʻia i loko o ka lolo a ʻo ka mea hope i hana ʻia i loko o ka lolo ma mua o ka hapa nui o nā kino ʻē aʻe.HANA E HOʻOʻOʻI: ʻO ka pōʻino neuronal a / a i ʻole demyelination ke kumu o ka piʻi ʻana o ka 24OHC mai ka lolo a i ka wai cerebrospinal (CSF), akā ʻo kahi pale koko-lolo ke kumu i hoʻonui ʻia ka flux o 27OHC mai ke kahe ʻana i ka CSF.Isotope dilution-mass. Ua hoʻohana ʻia ka spectrometry no ka hoʻāʻo ʻana i nā oxysterols ʻelua i CSF a me ka plasma mai nā maʻi ma mua o 250 me nā maʻi neurological a me geriatric like ʻole.ʻO nā maʻi me nā maʻi demyelinating ikaika ua hoʻonui i nā pae o 24OHC i CSF me kahi kiʻekiʻe 24OHC / 27OHC ratio.ʻO nā maʻi me ka maʻi maʻi maʻamau i loaʻa nā pae kiʻekiʻe o nā steroid ʻelua me kahi ratio haʻahaʻa 24OHC/27OHC.ʻO ka poʻe maʻi me ka maʻi Alzheimer ua hoʻonui iki i nā pae o 24OHC i CSF me ka liʻiliʻi o 27OHC.ʻO ka poʻe maʻi me ka nui o ka sclerosis i loaʻa i nā kiʻekiʻe kiʻekiʻe o 24OHC i nā wā hana me ka ratio 24OHC / 27OHC kiʻekiʻe. ʻO nā ana o nā oxysterols ʻelua i CSF a me ka plasma hiki ke hoʻohui nui i nā ʻano biochemical i loaʻa no ka loiloi ʻana i nā maʻi neurological.