Guanidine Hydrochloride Cas: 50-01-1 99% Keʻokeʻo pauka
| Helu Helu | XD90198 |
| inoa mea kūʻai | ʻO Guanidine Hydrochloride |
| CAS | 50-01-1 |
| ʻĀpana Molekala | CH6N3Cl |
| Kaumaha Molecular | 95.5388 |
| Nā kikoʻī mālama | Ambient |
| Kānāwai Kūʻai Kūlike | 29252900 |
Huahana Huahana
| Lae hehee | 185.3 - 187.5 Deg C |
| Wai | <0.3% |
| Ka hikiwawe | Hoʻomaʻemaʻe kala kala ʻole ma 6M i ka wai |
| Hoʻāʻo | >99% |
| UV Absorbance | 0.10 ma 260nm |
| Ka nana aku | Pauda keʻokeʻo |
| IR spectrum | Kūlike |
ʻO kekahi o nā kumu e pale ai i ka noi ʻana o ka lāʻau lapaʻau regenerative i nā maʻi degenerative cartilage he kumu kūpono o nā cell.ʻO Chondrocytes, ʻo ia wale nō ke ʻano cell cartilage, i ulu i loko o ka vitro ma lalo o nā kūlana moʻomeheu e hoʻonui i nā helu cell e nalowale i kā lākou phenotype me ka hiki ke hana i ka ʻiʻo cartilaginous hyaline.I loko o kēia noiʻi ʻana mākou e hoʻoholo ai e hoʻihoʻi hou ka ʻōnaehana moʻomeheu serum-a me ka ulu ʻole ʻekolu-dimensional (3D) i ka hiki o nā chondrocytes passaged e hana i ka ʻiʻo cartilage in vitro, kahi hana e pili ana i nā sox9.Bovine articular chondrocytes ua hele ʻelua i ka. e ʻae i ka hoʻonui ʻia ʻana o ka helu kelepona (P2) a hoʻomaʻamaʻa ʻia ma ke kiʻekiʻe kiʻekiʻe ma 3D collagen-type-II-coated membranes i loko o ka media content glucose kiʻekiʻe i hoʻohui ʻia me ka insulin a me ka dexamethasone (SF3D).Hōʻike ʻia nā cell ma hope o ka hoʻonui ʻana o ka monolayer a me ka hahai ʻana i ka moʻomeheu 3D e ka cytometry kahe, hōʻike gene, a me ka histology.Ua hōʻike ʻia nā hoʻololi mua i nā ala transduction hōʻailona i ka wā reddifferentiat ion. Ua hōʻike ʻia nā cell P2 i kahi progenitor-like antigen profile o 99% CD44 (+) a me 40% CD105 (+) a me kahi moʻolelo hōʻike gene e hōʻike ana i nā pūnaewele interzone.P2 ma SF3D i hōʻike i nā genes chondrogenic a hōʻiliʻili i ka matrix extracellular.Hoʻohaʻahaʻa i ka insulin receptor (IR) me HNMPA-(AM3) a i ʻole ke ala PI-3/AKT kinase (hoʻāla ʻia e ka mālama ʻana i ka insulin) me Wortmannin i kāohi i ka synthesis collagen.HNMPA-(AM3) hōʻemi hōʻike o Col2, Col11, a me IR genes a me Sox6 a me -9.Ua hōʻike ka co-immunoprecipitation a me ka chromatin immunoprecipitation anana o HNMPA-(AM3)-treated cell i ka hoʻopaʻa ʻana o nā coactivators Sox6 a me Med12 me Sox9 akā ua hoʻemi ʻia ʻo Sox9-Col2a1 binding. ka hoʻokumu ʻana o ka ʻiʻo cartilage e like me ka hyaline e ka paʻa ʻana o Sox9-Col2a1 i hoʻopili ʻia e ka insulin.ʻO ke kūpono o ka ʻiʻo i hana ʻia ma o kēia ala no ka hoʻohana ʻana i ka hoʻoponopono hui pono e nānā ʻia ma vivo.
