Hygromycin B CAS:31282-04-9 Buff pauka
Helu Helu | XD90374 |
inoa mea kūʻai | ʻO Hygromycin B |
CAS | 31282-04-9 |
ʻĀpana Molekala | C20H37N3O13 |
Kaumaha Molecular | 527.52 |
Nā kikoʻī mālama | 2 a 8 °C |
Kānāwai Kūʻai Kūlike | 2941900000 |
Huahana Huahana
Nā metala kaumaha | 20mg/kg max |
pH | 7-9.5 |
Hoʻāʻo | 99% |
Nalo ma ka maloo | 5% ka nui |
hana | 950u/mg min |
Ammonium | 1% ka nui |
Lehu hū | 5% ka nui |
Ka nana aku | Puka pauda |
Maemae TLC | >90% |
Hoʻohana mau ʻia ka wā kahiko o nā pūnae hū ma ke ʻano he kumu hoʻohālike no ka ʻelemakule o nā cell post-mitotic kanaka.ʻO ka hū Saccharomyces cerevisiae i ulu ma ka glucose i mua o ka ammonium sulphate i hoʻohana nui ʻia i ka noiʻi hū.Ua nānā mākou i ka wā kahiko o ka hū Hansenula polymorpha i ulu i nā kūlana e koi ai i ka metabolism peroxisome mua no ka ulu. ʻaʻole pono i nā peroxisomes.ʻO ke ola pōkole o H. polymorpha ma ka glucose ka mea nui ma muli o ka acidification medium, akā ʻo ka hapa nui o ka ROS ʻaʻole i hana koʻikoʻi.ʻO ka ulu ʻana o nā pūnaewele ma ka methanol/methylamine ma kahi o ka methanol/ammonium sulphate i hoʻonui i ke ola.ʻAʻole pili kēia i ka acidification medium.Hōʻike mākou i ka oxidation o ka methylamine e ka peroxisomal amine oxidase i nā kūlana pōloli kalapona ke kuleana no ka hoʻonui ʻana i ke ola.ʻO ka methylamine oxidation product formaldehyde ka mea i hoʻomaʻamaʻa hou ʻia i ka hopena o ka NADH generation, ka mea e hoʻonui ai i ka hoʻonui ʻana o ka ATP a me ka hōʻemi ʻana o nā pae ROS i ka manawa paʻa.Eia kekahi, ʻo ka hiki ke hana i ka NADH i nā kūlana pōloli kalapona e kahi kumu nitrogen organik e kākoʻo i ka hoʻonui hou ʻana i ke ola o ke kelepona.No laila, ʻo ka wehewehe ʻana i nā loiloi CLS i ka hū e pono e hoʻokomo i nā hopena i hiki i ke kūlana ikehu o ke kelepona.