Nā huahana

ʻO Adenosine kahi mea hoʻoponopono endogenous o ka leo vascular.Hoʻopau ʻia kēia hana o ka adenosine e kona lawe ʻana a me ka metabolism e nā cell endothelial microvascular (MVEC).ʻO ka transporter nui i komo ʻo ENT1 (equilibrative nucleoside transporter subtype 1).Hōʻike pū ʻia ʻo MVEC i ka nucleobase transporter (ENBT1) i komo i loko o ka cellular flux o adenosine metabolites e like me hypoxanthine.ʻO nā hoʻololi i kekahi o kēia mau ʻōnaehana kaʻa ka hopena i ka bioactivity o adenosine a me kāna metabolism, me ka hoʻokumu ʻana o nā radical manuahi.ʻO ka MVEC i hoʻokaʻawale ʻia mai ka ʻiʻo skeletal o ENT1 (+/+) a me ENT1 (-/-) nā ʻiole i hoʻokau ʻia i ke koʻikoʻi oxidative i hoʻoulu ʻia e ka ischemia / reperfusion a i ʻole menadione.Ua loiloi ʻia nā hana hana o ENT1 a me ENBT1 ma muli o ka zero-trans influx kinetics o nā substrates radiolabeled.Ua emi iho ka nui o ka ENBT1-mediated hypoxanthine uptake e ENT1(+/+) MVEC i mālama ʻia me ka menadione a i ʻole ma hope o ka ʻike ʻana i nā kūlana e hoʻohālike i ka isc hemia/reperfusion.Ma nā hihia ʻelua, ua hoʻopau ka superoxide dismutase mimetic MnTMPyP i ka nalowale o ka hana ENBT1, e pili ana i nā radical superoxide i ka pane.I ka hoʻokaʻawale ʻana, ʻaʻole i hōʻemi ʻia ka ʻiole ENT1 (-/-) MVEC i ka hana ENBT1 ma ka mālama ʻana me menadione a i ʻole ischemia / reperfusion i hoʻohālikelike ʻia, akā loaʻa iā lākou kahi kiʻekiʻe kiʻekiʻe o ka hana catalase e pili ana i ka ENT1 (+/+) MVEC.Hōʻike kēia mau ʻikepili e hoʻemi ʻia ka hana ENBT1 ma MVEC i ka pane ʻana i ka radical superoxide i hoʻonui ʻia e pili ana me ka ischemia / reperfusion injury.ʻAʻole hōʻike ka MVEC i hoʻokaʻawale ʻia mai nā ʻiole ENT1(-/-) i kēia hōʻemi i ka ENBT1, ma muli paha o ka hoʻonui ʻana i ka hana catalase.