L-Asparagine anhydrous CAS: 70-47-3 99% Ka pauka keʻokeʻo
| Helu Helu | XD90314 |
| inoa mea kūʻai | D-ʻakika Aspartic |
| CAS | 70-47-3 |
| ʻĀpana Molekala | C4H8N2O3 |
| Kaumaha Molecular | 132.12 |
| Nā kikoʻī mālama | Ambient |
| Kānāwai Kūʻai Kūlike | 29241900 |
Huahana Huahana
| Ka nana aku | Pauda keʻokeʻo |
| Hoʻāʻo | 99% |
| Kahuli kiko'ī | +34.2 a i +36.5 |
| Nā metala kaumaha | <10ppm |
| AS | <1ppm |
| pH | 4.4 - 6.4 |
| SO4 | <0.020% |
| Fe | <10ppm |
| Nalo ma ka maloo | <0.5% |
| NH4 | <0.10% |
| Cl | <0.020% |
| Mokuaina o ka hooponopono | >98% |
Hoʻopuka ʻia ʻo MSMEG_0307 ma ke ʻano he mea hoʻoponopono transcriptional no ka ʻohana protein AraC a aia ma kahi kokoke i ka moʻo N-acetyltransferase (nat) arylamine i Mycobacterium smegmatis, i loko o kahi puʻupuʻu gene, mālama ʻia i ka hapa nui o nā ʻano mycobacterial kaiapuni.I mea e wehewehe ai i ka hana o ka protein AraC mai ka nat operon ma M. smegmatis, ua ʻike ʻia ʻelua mau kumu DNA palindromic i mālama ʻia me ka bioinformatics a hoʻāʻo ʻia no ka hoʻopaʻa ʻana i ka protein me ka hoʻohana ʻana i ka electrophoretic mobility shift assays me kahi ʻano recombinant o ka protein AraC.Ua ʻike mākou i ka hoʻokumu ʻia ʻana o kahi DNA: AraC protein complex me kekahi o nā kumu a me ke ʻano o kēia kumu ma 20 loci ma waena o ka genome holoʻokoʻa o M. smegmatis, e kākoʻo ana i ka noho ʻana o kahi regulon mana AraC.No ka ʻike ʻana i nā hopena o AraC i ka hoʻoponopono ʻana i nā genes nat operon, a me ka loaʻa ʻana o ka ʻike hou aku i kāna hana, ua hana mākou i kahi ΔaraC mutant strain kahi i hoʻololi ʻia ai ka gene araC e kahi marker resistance hygromycin.ʻO ke kiʻekiʻe o ka hōʻike ʻana o nā genes nat a me MSMEG_0308 i hoʻoponopono ʻia i ka ΔaraC strain ke hoʻohālikelike ʻia me ke ʻano ʻano hihiu e hōʻike ana i kahi hopena hoʻoulu o ka protein AraC ma ka hōʻike ʻana o nā genes operon nat.
