Nā huahana

ʻO ke kumu nui o ka pilikia no ka maʻi Clostridium difficile (CDI) ʻo ia ka hoʻohana ʻana i nā lāʻau lapaʻau ma muli o ka hoʻopau ʻana i ka equilibrium o ka microbiota gut host.No ka mālama ʻana i ka hua bacteria probiotic i ka wā o ka mālama ʻana i ka maʻi maʻi, ʻo ka hoʻohana ʻana i nā molekala me ka hana antibacterial koho e hoʻonui i ka pono ma o ka wehe ʻana i ka C. difficile.ʻO kekahi o lākou ka mea kanu alkaloid 8-hydroxyquinoline (8HQ), ka mea i hōʻike ʻia e koho koho i ka clostridia me ka ʻole repressing bifidobacteria.Hoʻāʻo pinepine ʻia ka hana antimicrobial koho e nā ʻenehana moʻomeheu o nā maʻi bacterial pākahi.Eia nō naʻe, ʻo ka palena nui o kēia mau ʻenehana ʻo ka hiki ʻole ke wehewehe i ka ulu ʻana o ka ulu ʻana o nā maʻi bacterial hou aʻe i ka co-culture i loko o ka hoʻokolohua like.Ma ke aʻo ʻana i kēia manawa, ua hui mākou i ka fluorescent in situ hybridization a me ka cytometry kahe e wehewehe i nā loli i nā mea hana a me nā mea hana ʻole o kahi moʻomeheu hui i hana ʻia e ka pathogen C. diff icile CECT 531 a me ka Bifidobacterium longum subsp.longum CCMDMND BL1 ma hope o ka ʻike ʻana i 8HQ.Ua ʻike ʻia me ka ʻole o 8HQ, ʻaneʻane like ka like ʻana o nā kaha ʻelua, oscillating ma waena o 22.7 a me 77.9% i ka manawa o 12 h, akā me 8HQ ua emi iho ka hapa o ka C. difficile ikaika ma hope o 4 h, a hoʻomau wale ma waena o 8.8. a me 17.5 %.I ka hoʻohālikelike, ʻaʻole i hoʻopilikia ʻia ka ulu bifidobacterial e 8HQ.Ua hōʻike nā hopena o kēia noiʻi i ka hopena inhibitory koho o 8HQ ma ka clostridial a me ka bifidobacterial ulu ulu lāʻau, a me ka hiki o kēia pūhui no ka hoʻomohala ʻana i nā mea koho koho e hoʻomalu i nā CDI.