Nā huahana

ʻO ia ka EAAT2 glutamate transporter, helu no> 90% o ka hippocampal glutamate uptake.ʻOiai ʻo EAAT2 ka mea i hōʻike nui ʻia i nā astrocytes, ~ 10% o nā molekala EAAT2 i loaʻa i nā axon terminals.ʻOiai ka haʻahaʻa haʻahaʻa o ka ʻōlelo EAAT2 i nā glutamatergic terminals, i ka wā e hoʻomoʻi ʻia ai nā ʻāpana hippocampal me ka haʻahaʻa haʻahaʻa o ka d-aspartate (kahi substrate EAAT2), e hōʻiliʻili nā pahu axon i ka d-aspartate e like me ka astroglia.Hōʻike kēia i kahi mismatch i wehewehe ʻole ʻia ma waena o ka hāʻawi ʻana i ka protein EAAT2 a me ka hana lawe kaʻa ʻana o EAAT2.ʻO kekahi kuhiakau, ʻo (1) ʻoi aku ka wikiwiki o ka hoʻololi ʻana o ka substrate i loko me ka substrate o waho ma mua o ka lawe ʻana i ka ʻupena a (2) makemake nā mea hope i ka heteroexchange ma muli o ke kiʻekiʻe o ka glutamate kūloko.Eia nō naʻe, ʻaʻole i ʻike ʻia i kēia manawa inā like a like ʻole ka heteroexchange a me ka uptake.No ka hoʻoponopono ʻana i kēia pilikia, ua hoʻohana mākou i kahi ʻōnaehana hou e hoʻohālikelike i nā helu pili o nā kaʻina hana ʻelua i ka ʻiole a me ka ʻiole.Ua ʻike ʻia ka uptake Net i nā loli i ka hiki o ka membrane a ua hoʻoulu ʻia e nā anion permeable waho e like me ke ʻano o kahi conductance anion uncoupled.Ma ka hoʻohana ʻana i ka mea hope, hōʻike pū mākou e pili ana ka nui o ka heteroexchange i ka hiki ke membrane.Hoʻohui hou, hōʻike hou kā mākou ʻikepili i ka hiki ʻana o kahi leak sodium ma EAAT2.Ma ka hoʻokomo ʻana i nā ʻike hou i kā mākou kumu hoʻohālike mua o ka glutamate uptake e EAAT2, wānana mākou i ka ʻike ʻana o ka volta o ka hoʻololi ʻana ma muli o ka hoʻopaʻa ʻana o ke kolu o Na (+).Eia hou, hōʻike kā mākou mau hoʻokolohua a me nā hoʻohālikelike e hoʻohālikelike ʻia nā helu pili o ka uptake uptake a me ka heteroexchange ma EAAT2.