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ʻO ka hōʻiliʻili nukili o kahi ʻano mutant o ka protein nuklea Lamin-A, i kapa ʻia ʻo Progerin (PG) a i ʻole Lamin AΔ50, loaʻa ma Hutchinson-Gilford Progeria Syndrome (HGPS) a i ʻole Progeria, kahi maʻi ʻelemakule wikiwiki.ʻO kekahi o nā hōʻailona nui o kēia maʻi genetic ka nalowale o ka momona subcutaneous ma muli o kahi lipodystrophy dramatic. Ua hoʻoikaika mākou i ka hōʻike ʻana o ke kanaka PG a me GFP -Green Fluorescent Protein- ma ke ʻano he mana i nā cell 3T3L1 me ka hoʻohana ʻana i kahi ʻōnaehana lentiviral e aʻo i ka ka hopena o ka hōʻike PG i ka mana ʻokoʻa o kēia laina kelepona, kekahi o nā hiʻohiʻona adipogenic i hoʻohana nui ʻia.Ua hana ʻia ka quantitative proteomics (iTRAQ) e aʻo i ka hopena o ka hōʻiliʻili PG.Ua hōʻoia ʻia kekahi o nā protein modulated e immunoblotting a me PCR manawa maoli.Ua kālailai ʻia ka hana mitochondrial ma ke ana ʻana o a) ka hana basal mitochondrial, b) ka hana ʻana o ka anion superoxide a me c) ka pono o kēlā me kēia kanaka o ka paʻakikī like ʻole o ke kaulahao hanu. ka emi ʻana o ka proliferation rate a me nā hemahema i ka mana adipogenic ke hoʻohālikelike ʻia i ka mana.Ua hōʻike ʻia ka nui o ka proteomics analysis i nā proteins 181 me ka nui (p<0.05) i hoʻololi ʻia i nā preadipocytes e hōʻike ana i ka PG.Hoʻopilikia ʻia ka hana Mitochondrial i nā pūnaewele hōʻike PG.Ma keʻano kikoʻī, ua ʻike mākou i ka piʻi ʻana o ka hana o ka complex I a me ka overproduction o Superoxide anion.ʻO ka incubation me ka Reactive Oxygen Species (ROS) scavenger agents e hoʻoemi i ka autophagic proteolysis e like me ka mea i hōʻike ʻia e LC3-II/LC3-I ratio. ka hoʻoponopono, ka unuhi ʻana, ka pelu ʻana i ka protein a me ka hana ʻana o ka ikehu e ka mitochondria.Hoʻoikaika kā mākou ʻikepili i ka hāʻawi ʻana o ka hōʻiliʻili ROS i ka phenotype ʻelemakule mua a hoʻokumu i kahi loulou ma waena o ka mitochondrial dysfunction a me ka nalowale o ka proteostasis ma HGPS.