L-Methionine Cas: 63-68-3 99% Keokeo crystalline pauka
| Helu Helu | XD90287 |
| inoa mea kūʻai | L-Methionine |
| CAS | 63-68-3 |
| ʻĀpana Molekala | C5H11NO2S |
| Kaumaha Molecular | 149.21134 |
| Nā kikoʻī mālama | Ambient |
| Kānāwai Kūʻai Kūlike | 29304010 |
Huahana Huahana
| Hoʻāʻo | 98% - 101.5% |
| Ka nana aku | ʻO nā kristal keʻokeʻo a i ʻole ka pauka crystalline |
| Ka hopena | |
| ʻIkepili | Hoʻokō i ke koi |
| pH | 5.6 - 6.1 |
| Nalo ma ka maloo | ≤ 0.3% |
| Sulfat (SO4) | ≤ 0.03% |
| hao | ≤ 0.003% |
| Koena ma ka Ignition | ≤ 0.4% |
| Klorida | ≤ 0.05% |
| Metala Kaumaha | ≤ 0.0015% |
| Maemae Chromotograhic | ʻAʻole ʻoi aku ma mua o 2.0% o ka nui o nā haumia i loaʻa |
| Ka hoʻololi kikoʻī [ α ] D 2 5 | +22.4º ~ +24.7º |
Ua hōʻike kā mākou mau haʻawina mua i kāna mau kuleana koʻikoʻi i ka wā mua a me ka hope o ka maʻi KSHV.Ma kēia haʻawina, ua nānā mākou i ka launa pū ʻana o ORF45 me ka hoʻohana ʻana i kahi papa o nā antibodies monoclonal.Ma kahi o ka extracellular regulated kinase (ERK) a me p90 ribosomal S6 kinase (RSK) proteins, ua loaʻa iā mākou kekahi mau protein copurified ʻē aʻe, me nā mea koʻikoʻi o ~38 kDa a me ~130 kDa.Ua hōʻike ʻia ka mass spectrometry ʻo ka protein 38-kDa he viral ORF33 a ʻo ka protein 130-kDa he cellular USP7 (ubiquitin-specific protease 7).Hoʻopili mākou i ka ORF33-binding domain i ka carboxyl-terminal 19 amino acids (aa) o ORF45 a me ka USP7-binding domain i ka motif consensus i hōʻike ʻia ma ke kikowaena o ORF45.Ke hoʻohana nei i ka immunofluorescence staining, ʻike mākou i ka colocalization o ORF45 me ORF33 a i ʻole USP7 ma lalo o nā kūlana i hoʻololi ʻia a i loko o nā cell i hoʻopili ʻia e KSHV.Eia kekahi, ua ʻike mākou i ka hoʻololi hou ʻana o ORF45 i kahi ʻāpana o ORF33/USP7 mai ka nucleus i ka cytoplasm.Ua ʻike mākou ua hoʻonui ʻo ORF45 i ka hōʻiliʻili protein ORF33 i hoʻopau ʻia inā i holoi ʻia ka ORF33- a i ʻole USP7-binding domain ma ORF45.Eia kekahi, ʻo ka holoi ʻana i ka pauku carboxyl i mālama ʻia o ORF45 i loko o ka genome KSHV i hoʻemi nui i ke kiʻekiʻe o ka protein ORF33 i loko o nā cell i hoʻopili ʻia e KSHV a hoʻopau i ka hana ʻana o nā virion progeny.ʻO ka hui pū ʻana, ʻaʻole hōʻike wale kā mākou hopena i nā mea hou o ka ORF45 interactome, akā hōʻike pū kekahi i ka pilina ma waena o kēia mau protein he mea koʻikoʻi no ka KSHV lytic replication. ʻO Kaposi's sarcoma-associated herpesvirus (KSHV) ke kumu kumu o kekahi mau maʻi maʻi kanaka.ʻO KSHV ORF45 he pūmua multifunctional i koi ʻia no ka hoʻopiʻi lytic KSHV, akā ʻaʻole maopopo nā ʻano hana pololei e hana ai ʻo ORF45 i kāna mau hana koʻikoʻi.Ua hōʻike ʻia kā mākou mau noiʻi mua ʻana aia nā pūmua ORF45 āpau i nā cell i loko o nā paʻakikī kiʻekiʻe-molecular-weight.No laila ua ʻimi mākou e ʻike i ka launa pū ʻana o ORF45 e hāʻawi i nā ʻike i kāna mau kuleana i ka wā o ka lytic replication.Ke hoʻohana nei i kahi papa o nā antibodies monoclonal, ua nānā mākou i ka ORF45 interactome i loko o nā pūnaewele i hoʻopili ʻia e KSHV.Ua ʻike mākou i ʻelua mau hoa paʻa hou o ORF45: ʻo ka protein viral ORF33 a me ka protease ubiquitin-specific 7 (USP7).Ke hōʻike hou aku nei mākou he mea koʻikoʻi ka pilina ma waena o ORF45 a me ORF33 no ka hana maikaʻi ʻana o nā ʻāpana viral KSHV, e hōʻike ana i ka hoʻopili ʻia ʻana o kēia pilina e hōʻike i kahi hoʻolālā hou e kāohi ai i ka replication lytic KSHV.
