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Hoʻopilikia ʻo MES Buffer i ka Arabidopsis Root Apex Zonation a me Root Growth ma ke kāohi ʻana i ka Superoxide Generation ma Root Apex

I loko o nā mea kanu, hoʻoponopono ʻia ka ulu ʻana o nā aʻa a me nā lauoho aʻa e ka mana kelepona maikaʻi o ka pH a me nā ʻano oxygen reactive (ROS).MES, 2-(N-morpholino)ethanesulfonic acid e like me kekahi o ka Good's buffers ua hoʻohana nui ʻia no ka buffering medium, a ua manaʻo ʻia he kūpono ia no ka ulu ʻana o nā mea kanu me ka hoʻonui ʻana ma 0.1% (w/v) no ka mea hiki ke hoʻopaʻa ʻia o MES mai ka pH 5.5-7.0 (no Arabidopsis, pH 5.8).Eia naʻe, nui nā hōʻike i hōʻike ʻia, ma ke ʻano, pono nā aʻa i nā waiwai pH like ʻole ma ka ʻili o nā ʻāpana apex aʻa, ʻo ia hoʻi meristem, wahi hoʻololi, a me kahi elongation.ʻOiai ʻo ka ulu mau ʻana o nā aʻa ma luna o kahi media e loaʻa ana i ka molekele buffer, ʻike liʻiliʻi e pili ana i ka hopena o MES i ka ulu ʻana o ke aʻa.Maʻaneʻi, ua nānā mākou i nā hopena o nā manaʻo likeʻole o ka MES buffer e hoʻohana ana i nā aʻa ulu o Arabidopsis thaliana.Hōʻike kā mākou mau hopena i ka 1% o MES i kaohi nui i ka ulu ʻana o ke aʻa, ka helu o nā lauoho aʻa a me ka lōʻihi o ka meristem, ʻoiai ʻo 0.1% i hāpai i ka ulu ʻana o ke aʻa a me ka ʻāpana apex aʻa (ka ʻāina mai ke kumu aʻa a hiki i ka wahi hoʻololi).Eia kekahi, ua nalowale ka hana superoxide ma ka aʻa aʻa ma 1% o MES.Hōʻike kēia mau hopena e hoʻopilikia ka MES i ka morphogenesis maʻamau ma o ka hoʻololi ʻana i ka homeostasis ROS ma ke aʻa aʻa.