Nā huahana

Ua wehewehe ʻia kahi kaʻina hana immunoassay solid-phase no ka hoʻoholo ʻana i ka progesterone i loko o ka plasma kanaka, e hoʻohana ana i ka chemiluminescence ma ke ʻano he hopena.Hoʻohana ʻia kahi conjugate progesterone-isoluminol ma ke ʻano he māka chemiluminescent.Hoʻopili ʻia kahi hapa IgG o ka antiserum i ka progesterone-11 alpha-hemisuccinate bovine serum albumin i nā paia o nā paipu hoʻāʻo polystyrene Lumacuvettes P.Ma hope o ka hoʻopaʻa ʻana, holoi ʻia nā cuvettes.ʻO ka hāʻawi ʻana o ka māmā o ka conjugate i hoʻopaʻa ʻia ma luna o ka oxidation me kahi ʻōnaehana H2O2-microperoxidase ma ka pH 13 ua hoʻemi ʻia me ka hoʻonui ʻana i ka ʻike progesterone manuahi ma ke ʻano log-linear ma luna o ka laulā 15-1000 pg/tube.Hoʻohālikelike ʻia ka immunoassay chemiluminescence me ka radioimmunoassay e pili ana i ka ʻike, kikoʻī, pololei a me ka pololei.Hāʻawi ka assay i nā pono o ka wikiwiki a me ka maʻalahi o ka automation.Eia kekahi, ʻaʻole pili kēia ʻano i ka hoʻohana ʻana i nā radioisotopes a i ʻole ka centrifugation step.