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Nui nā poʻe koa aloha ʻole a puni mākou, he mea maʻamau, akā ʻoiaʻiʻo, hāʻawi nui lākou iā mākou.ʻO Proteinase K ka "hero unsung" i ka ʻoihana diagnostics molecular, ʻoiai ke hoʻohālikelike ʻia me ka "nui a ikaika" i ka ʻoihana, ʻo ka proteinase K he haʻahaʻa haʻahaʻa a ua lōʻihi mākou i nānā ʻole i kona koʻikoʻi.Me ka puka ʻana o ka maʻi lei aliʻi hou, ua piʻi nui ka noi no ka proteinase K, a ʻo ka lako ma ka home a me waho ma hope o ka ʻai ʻana, a ʻike koke nā mea āpau he mea nui ka proteinase K.
He aha ka hoʻohana ʻana o ka proteinase K
ʻO Proteinase K he protease serine me ka hana enzyme proteolytic a hiki ke mālama i ka hana ma kahi ākea o nā kaiapuni (pH (4-12.5), paʻakai paʻakai kiʻekiʻe, kiʻekiʻe kiʻekiʻe o 70 ° C, etc.).Eia kekahi,ʻaʻole i kāohiʻia ka hana o ka proteinase K e SDS, urea, EDTA, guanidine hydrochloride, guanidine isothiocyanate, a me nā mea'ē aʻe, a me kekahi nui o ka mea holoi hiki ke hoʻonui i ka hana o ka proteinase K. I ka lāʻau lapaʻau (virus and microbial disinfection). ), ka meaʻai (ka hoʻomaʻamaʻa ʻana i ka ʻiʻo), ka ʻili (hoʻomaʻamaʻa lauoho), ka hana waina (ka hoʻomaʻamaʻa ʻana i ka waiʻona), ka hoʻomākaukau ʻana o ka waikawa amino (nā hulu i hoʻohaʻahaʻa ʻia), ka ʻokiʻoki nucleic acid, in situ hybridization, etc., proteinase K Aia nā noi.ʻO ka noi maʻamau i hoʻohana pinepine ʻia ʻo ia ka nucleic acid extraction.
Hiki iā Proteinase K ke enzymolyze i nā ʻano protein a pau i loko o ka hāpana, me kēlā mau histones i hoʻopaʻa paʻa ʻia i nā ʻakika nucleic, i hiki ke hoʻokuʻu ʻia nā ʻakika nucleic mai ka hāpana a hoʻokuʻu ʻia i loko o ka extract, e hoʻomaʻamaʻa i ka hana aʻe o ka unuhi a me ka hoʻomaʻemaʻe.I ka ʻike ʻana i ka waikawa nucleic viral, ʻo ka proteinase K kekahi o nā mea koʻikoʻi i ka hopena hoʻohālike virus.Hiki i ka Proteinase K ke uhae a hoʻopau i ka protein coat o ka maʻi, ʻoi aku ka palekana i ka wā o ka lawe ʻana a me ka ʻike;Eia kekahi, hiki i ka proteinase K ke hoʻohaʻahaʻa i ka RNase e pale i ka hōʻino ʻana o ka RNA viral a hoʻomaʻamaʻa i ka ʻike nucleic acid.
ʻO ka kaulana i ka pō o ka proteinase K
Inā ma ke kahua o ka noiʻi ʻepekema a i ʻole ma ke kahua o IVD, ʻo ka ʻili ʻana o ka nucleic acid ka hoʻokolohua maʻamau, no laila he mea nui loa ka proteinase K.Eia naʻe, i ka wā ma mua, ʻaʻole i ʻike nui ʻia ka proteinase K ma mua o kāna kuleana.ʻO kahi hapa nui o kēia no ka mea paʻa loa ka pilina hāʻawi a me ke koi o ka proteinase K.He kakaikahi ka poʻe e manaʻo he pilikia ka hāʻawi ʻana i ka proteinase K.
Me ka puka ʻana o ke ahulau lei hou, ua piʻi ka noi no ka hoʻāʻo ʻana i ka nucleic acid.Ma ka hopena o Iune 2020, ua hoʻopau ʻo Kina ma kahi o 90 miliona mau hoʻokolohua lei aliʻi hou, a ʻoi aku ka weliweli o kēia helu ma ka pae honua.I nā hoʻokolohua hoʻokolohua nucleic acid extraction, ʻo ka ʻike hana o ka proteinase K ma kahi o 50-200 μg/mL.ʻO ka maʻamau, pono ia ma kahi o 100 μg o ka proteinase K e unuhi i kahi laʻana o ka waikawa nucleic.I ka hoʻohana maoli ʻana, i mea e hoʻonui ai i ka pono o ka unuhi ʻana o ka nucleic acid, e hoʻohana pinepine ʻia ʻo Proteinase K i ka nui o ka nui.ʻO ka ʻike ʻana i ka waikawa nucleic o ka coronavirus hou i lawe mai i ka nui o ka proteinase K koi.Ua wāwahi koke ʻia ka hoʻolako ʻana a me ke koena koi o ka proteinase K, a ua lilo ka proteinase K i mea nui e pale ai i ka maʻi ahulau i ka pō.
ʻO nā pilikia i ka hana ʻana o ka proteinase K
ʻOiai me ka ulu ʻana o ka maʻi ahulau, ua waiwai nui ʻia ka waiwai nui o ka proteinase K e nā kānaka, he mea hilahila ma muli o ka haʻahaʻa haʻahaʻa haʻahaʻa o ka proteinase K, liʻiliʻi nā hui kūloko i komo i ka hana o ka proteinase K. makemake e hoʻokumu i ka hana proteinase K I ka wā o ke kaʻina hana, ua ʻike ʻia ʻo ka proteinase K he protein kūikawā kūikawā.He mea paʻakikī loa ka hoʻonui ʻana i ka mana hana o ka proteinase K i kahi manawa pōkole.
ʻO ka hana nui o ka proteinase K ke kū nei i nā pilikia e hiki mai ana
1. ʻŌlelo haʻahaʻa
Hiki i ka Proteinase K ke hoʻohaʻahaʻa pono ʻole i ka hapa nui o nā protein a hoʻoulu i nā mea ʻawaʻawa nui i ka cell host expression.No laila, haʻahaʻa loa ka pae ʻōlelo o ka proteinase K.ʻO ka nānā ʻana i nā ʻōnaehana hōʻike a me nā kānana e hōʻike ana i ka proteinase K maʻamau e koi i kahi pōʻai lōʻihi.
2. Ke koena o nā puaʻa a me nā ʻakika nucleic
Hoʻokomo ka fermentation nui i ka nui o ka puaʻa a me nā koena nucleic acid.He mea paʻakikī ke wehe i kēia mau haumia me kahi kaʻina hoʻomaʻemaʻe maʻalahi, a hoʻonui ka hoʻomaʻemaʻe paʻakikī i ke kumukūʻai a hoʻemi i ka helu hoʻihoʻi.
3. Hiki ole
ʻAʻole paʻa ka Proteinase K, hiki iā ia ke enzymolyze iā ia iho, a paʻakikī ke mālama pono iā ia ma 37 ° C no ka manawa lōʻihi me ka ʻole o kahi mea pale.
4. He maʻalahi ke hoʻoheheʻe
I ka hoʻomākaukau ʻana i ka pauka maloʻo maloʻo o ka proteinase K, i mea e hōʻoia ai i ka nui o ka mea paʻa o ka proteinase K i loko o ka pauka maloʻo maloʻo, pono e hoʻohui i kahi mea pale maloʻo maloʻo ma kahi kiʻekiʻe, akā i ka wā ʻO ka nui o ka proteinase K hiki i ka 20mg / mL a ma luna, ua maʻalahi ka hoʻohui ʻana i kahi precipitate, kahi e lawe mai ai i nā pilikia nui i ka maloʻo maloʻo o ka proteinase K me kahi mea paʻa kiʻekiʻe.
5. Hoʻokomo kālā nui
Loaʻa i ka Proteinase K ka hana protease ikaika a hiki iā ia ke hydrolyze i nā protease ʻē aʻe i loko o ka hale hana.No laila, pono ka proteinase K i nā wahi hana kūikawā, nā mea hana, a me nā limahana no ka noiʻi a me ka hoʻomohala ʻana a me ka hana ʻana.
XD BIOCHEM's proteinase K solution
XD BIOCHEM has a mature protein expression and purification platform, and has rich experience in the expression and purification of recombinant proteins and optimization of production processes. Through the rapid formation of a research and development team, the large-scale production process of proteinase K has been overcome. The monthly output of freeze-dried powder is more than 30 KG. The product has stable performance, high enzyme specific activity, and no host cytochrome and nucleic acid residues. Welcome to contact XD BIOCHEM Obtain a trial package (E-mail: sales@xdbiochem.com Tel: +86 513 81163739).
XD BIOCHEM nā hoʻonā ʻenehana
Ke hoʻohana nei i ka hoʻohui ʻana i ka plasmid multi-copy, koho ʻia nā kaha kiʻekiʻe me ka pae hōʻike o 8g/L, e lanakila ana i ka pilikia o ka pae haʻahaʻa haʻahaʻa o ka proteinase K;
Ma o ka hoʻokumu ʻana i kahi kaʻina hoʻomaʻemaʻe multi-step, ua hoʻoneʻe maikaʻi ʻia ka cytochrome host a me nā koena nucleic acid o proteinase K ma lalo o ka waiwai maʻamau;
Ma o ke kiʻekiʻe-throughput screening o ka pale pale formulations, kahi paʻa hiki ke mālama pono i ka proteinase K ma 37 ° C i koho ʻia;
Ua lanakila nā pale pale pale i ka pilikia i maʻalahi ka proteinase K e hōʻuluʻulu a hoʻoheheʻe ʻia i nā kiʻekiʻe kiʻekiʻe, a waiho i ke kumu no ka proteinase K's high solid content freeze-drying.

XD BIOCHEM proteinase K hāpana

XD BIOCHEM proteinase K ho'āʻo paʻa: ʻaʻohe hoʻololi koʻikoʻi o ka hana ma hope o 80 d ma ka lumi wela

XD BIOCHEM proteinase K ho'āʻo paʻa: ʻaʻohe hoʻololi koʻikoʻi o ka hana ma hope o 80 d ma ka lumi wela

Ka hoʻohālikelike ʻana i ka hopena hoʻoemi nucleic acid o XD BIOCHEM proteinase K a me nā huahana hoʻokūkū.I ke kaʻina hana o ka nucleic acid extraction, hoʻohana ʻia ʻo XD BIOCHEM a me ka proteinase K hoʻokūkū i kēlā me kēia.ʻOi aku ka maikaʻi o ka unuhi ʻana o XD BIOCHEM proteinase K a ʻoi aku ka haʻahaʻa o ka waiwai Ct o ka gene i hoʻopaʻa ʻia.