Phosphatase, alkaline CAS:9001-78-9 keʻokeʻo, hina keʻokeʻo a i ʻole peach monoclinic crystal a pauda
| Helu Helu | XD90379 |
| inoa mea kūʻai | Phospatase, alkaline |
| CAS | 9001-78-9 |
| ʻĀpana Molekala | C21H36N8O6 |
| Kaumaha Molecular | 496.57 |
| Nā kikoʻī mālama | 2 a 8 °C |
| Kānāwai Kūʻai Kūlike | 35079090 |
Huahana Huahana
| Hoʻāʻo | 99% |
| Ka nana aku | keʻokeʻo, hina keʻokeʻo a i ʻole peach monoclinic crystal a pauka paha |
ʻOiai ua ʻike ʻia ka fluoride e hoʻoulu i ka hoʻokumu ʻana o ka iwi, ʻaʻole maopopo loa nā hana kumu.Ua hoʻokomo nā haʻawina hou i ke ala Wnt/β-catenin ma ke ʻano he cascade hōʻailona nui i ka biology iwi.Ua hōʻike ko mākou mau haʻawina mua i ke kuleana o ke ala Wnt canonical i ka hoʻokumu ʻana i nā iwi o nā ʻiole i hōʻike ʻia i ka fluoride mau loa, akā ʻaʻole maopopo ke ʻano.Ua hoʻoholo ka haʻawina o kēia manawa i ke komo ʻana o ka hōʻailona Wnt / β-catenin i ka ʻokoʻa osteoblastic i hoʻokomo ʻia i ka fluoride.Me ka hoʻohana ʻana i nā osteoblast iole mua, ua hōʻike mākou ua hoʻoikaika nui ka fluoride i ka hoʻonui ʻana o ka osteoblasts a me ka hōʻike alkaline phosphate (ALP) a me nā pae hōʻike mRNA o nā māka hoʻokaʻawale iwi, me ka type I collagen (COL1A1), ALP a me osteonectin.Ua ʻike hou mākou i nā phosphorylations i hoʻokomo ʻia i ka fluoride ma serine 473 o Akt a me serine 9 o glycogen synthase kinase-3β (GSK3β), i hopena i ka GSK-3β inhibition a ma hope o ka hōʻiliʻili ʻana o ka β-catenin, e like me ka hōʻike ʻana e Western blot a me immunofluorescence analysis. .Eia kekahi, ua hoʻokomo pū ka fluoride i ka ʻōlelo o Wnt-targeted gene runt-related transcription factor 2 (Runx2).ʻO ka mea nui, ua hoʻopau ʻia ka hopena maikaʻi o ka fluoride ma ka hana ALP a me nā hōʻike mRNA o COL1A1, ALP, osteonection a me Runx2 e DKK-1, kahi blocker o ka Wnt / β-catenin receptor.Hoʻohui pū ʻia, hōʻike kēia mau ʻike e hoʻoikaika ka fluoride i ka hoʻokaʻawale osteoblastic ma o ka hoʻāla ʻana o Akt- a me GSK-3β o ke ala hōʻailona Wnt/β-catenin i nā osteoblast iole mua.Hāʻawi kā mākou ʻike i nā ʻike hou i ka hana o ka fluoride i ka osteoblastogenesis.
