PIPES Cas: 5625-37-6 ʻO ka pauka crystalline keʻokeʻo 99% ABTS DIAMMONIUM SALT ULTRA PURE GRADE
| Helu Helu | XD90117 |
| inoa mea kūʻai | PIPES (Piperazine-1,4-bis(2-ethanesulfonic acid)) |
| CAS | 5625-37-6 |
| ʻĀpana Molekala | C8H18N2O6S2 |
| Kaumaha Molecular | 302.37 |
| Nā kikoʻī mālama | Ambient |
| Kānāwai Kūʻai Kūlike | 2933599 |
Huahana Huahana
| Nā metala kaumaha | <5ppm |
| Nalo ma ka maloo | <1.0% |
| Ka hikiwawe | ʻAkaka, ʻaʻohe kala ʻole (5% 1N NaOH) |
| Hoʻāʻo | 99 - 101% |
| Ka nana aku | ʻO ka pauka kristal keʻokeʻo |
ʻO PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] hoʻohana pinepine ʻia ma ke ʻano he mea hoʻopaʻa i ka biochemistry.He ethanesulfonic acid buffer i hoʻomohala ʻia e Good et al.i ka makahiki 1960.Loaʻa iā PIPES kahi pKa kokoke i ka pH physiological e pono ai i ka hana moʻomeheu cell.Ua kākau ʻia e hōʻemi i ka nalowale o ka lipid i ka wā e hoʻopaʻa ai i ka histology glutaraldehyde i nā mea kanu a me nā ʻiʻo holoholona.
Hoʻokumu ka Antimicrobial peptide magainin 2 i nā pores i loko o nā membrane lipid a hoʻoulu i ka membrane permeation o nā ʻike pūnaewele.ʻOiai ʻo kēia permeation ke kumu nui o kāna hana bactericidal, ʻaʻole maopopo ka ʻano o ka hana pore.No laila mākou i noiʻi kikoʻī i ka pili ʻana o ka magainin 2 me nā membrane lipid me ka hoʻohana ʻana i nā vesicle unilamellar nui (GUVs).ʻO ka hoʻopaʻa ʻana o ka magain 2 i ka membrane lipid o nā GUV i hoʻonui i ka hoʻololi fractional ma kahi o ka membrane, δ, i like me ka ʻili o ka ʻili o ka magainin 2, X. Hōʻike kēia i ka helu mau o ka magainin 2-induced ʻelua. - Ua hoʻonui nui ʻia ka hoʻololi ʻana mai ke kūlana paʻa i ka mokuʻāina pore me ka piʻi ʻana o δ.ʻO ka hoʻopaʻapaʻa ʻana o kahi membrane lipid ma hope o ka manaʻo ʻana o kahi GUV i hoʻāla pū ʻia i ka hoʻokumu ʻana o ka pore i hoʻokumu ʻia i ka magainin 2.No ka hōʻike ʻana i kahi o ka magainin 2, ua noiʻi ʻia ka launa pū ʻana o carboxyfluorescein (CF) -labeled magainin 2 (CF-magainin 2) me nā GUV hoʻokahi i loaʻa i ka wai-soluble fluorescent probe, AF647, me ka hoʻohana ʻana i ka microscopy confocal.I ka loaʻa ʻole o ka hoʻopaʻapaʻa ma muli o ka aspiration, ma hope o ka hoʻopili ʻana o ka magainin 2 i ka nui o ka fluorescence o ka GUV rim ma muli o CF-magainin 2 ua piʻi wikiwiki i kahi waiwai paʻa, i noho mau no ka manawa lōʻihi, a ma 4-32 s ma mua. ka hoʻomaka ʻana o ka leakage o AF647 ua hoʻomaka ka ikaika o ka rim e piʻi wikiwiki i kahi waiwai paʻa.I ka ʻokoʻa, i mua o ka ʻāʻī, ʻaʻohe piʻi o ka ikaika o ka rim ma mua o ka ʻike ʻia ʻana o ka leakage.Hōʻike kēia mau hopena ʻaʻole hiki i ka magainin 2 ke unuhi mai waho a i ka monolayer i loko a hiki i ka wā ma mua o ka hoʻokumu ʻana o ka pore.Ma muli o kēia mau hopena, ua hoʻoholo mākou he pore i hoʻokomoʻia i ka magainin 2 he pore i hoʻouluʻia aʻo ka hoʻonuiʻana o ka monolayer i loko he mea hoʻoikaika nui ia o ka hana pore.
