Nā huahana

ʻO kahi biosensor microRNA (miRNA) koʻikoʻi e hoʻohana ana i ka ruthenium oxide nanoparticle (RuO (2) NP) -i hoʻomaka i ka polymerization o 3,3'-dimethoxybenzidine (DB) a me ka miRNA-templated deposition o kahi poly insulating (3,3'-dimethoxybenzidine) (PDB) kiʻiʻoniʻoni i wehewehe ʻia ma kēia hana.Ua hana ʻia ka biosensor me kahi monolayer hui ʻia o nā oligonucleotide hopu probes (CPs) a me 4-mercaptoaniline ma kahi electrode gula.Ma hope o ka hoʻohuihui ʻana me kahi RuO(2) NP-tag target miRNA, ua hoʻopili ʻia ka hui ʻana o DB/H(2)O(2) i ka pH 5.0 0.10 M acetate buffer i ka biosensor.ʻO nā RuO (2) NP e lawelawe i ka polymerization initiator / catalyst no ka polymerization o DB.A ʻo nā kaula miRNA anionic hybridized a me nā CP manuahi e lilo i kumu hoʻohālike, e alakaʻi ana i ka waiho ʻana o PDB.ʻO ka nui o ka PDB i waiho ʻia a me kona mana insulating e hoʻopili pololei i ka neʻe ʻana o ka miRNA pahuhopu i ka hopena.Ua hōʻike ʻia nā hoʻāʻo spectroscopic impedance electrochemical i loaʻa ka pilina linear charge-transfer resistance-concentration mai 6 .0 fM a i 2.0 pM ma hope o 60 min o ka hoʻoulu ʻana i ka hui DB/H(2)O(2).ʻAʻohe cross-hybridization ma waena o ka pre-miRNA a me ka miRNA makua a me ka liʻiliʻi loa o ka cross-hybridization ma waena o nā lālā o ka ʻohana miRNA pili pili a hiki i nā pae hoʻokahi-base-mismatched.Hāʻawi kēia biosensor e pili ana i ka impedance i kahi koho maikaʻi no ka hōʻike hōʻike miRNA a hiki ke hiki ke hoʻomohala i kahi ʻōnaehana profiling miRNA multiplexing portable.