Nā huahana

Ua hana ʻia nā mea ʻike PH e ka anodically electrodepositing iridium oxide films (AEIROFs) ma luna o nā microelectrodes ma nā chips a uhi ʻia me ka poly(ethyleneimine) (PEI) no ka paʻa mechanical.Hōʻike kēia i ka pane super-Nernstian i ka pH mai ka pH 4.0 a i ka 7.7 i loko o ka chloride-free phosphate buffer.Ua uhi ʻia ka ʻili o ka puʻupuʻu me ka fibronectin no ka hoʻopili ʻana o nā pūnana endothelial porcine aortic (PAECs).Ua noiʻi ʻia ka hiki ke hana o ka sensor pH no ka nānā ʻana i nā loli pH kūloko ma o ka hoʻoulu ʻana i nā PAEC me ka thrombin.Hōʻike kā mākou hopena i ka thrombin i hoʻokomo i ka acidification extracellular acute o PAEC a me ka hoʻopau ʻana o ka fibronectin, e hoʻemi ana i ka pH kūloko.ʻO ka hoʻohana ʻana i ka PD98059, kahi mea hoʻokaʻawale mitogen-activated protein kinase (MAPK), hoʻemi i ka acidification extracellular a me ka piʻi ʻana o ka pH kūloko.Hōʻike kēia haʻawina e hiki i kā mākou mau mea ʻike pH ke hoʻomaʻamaʻa i ka hoʻokolokolo ʻana i nā pane cellular acute i ka hoʻoulu ʻana ma o ka nānā ʻana i ka manawa maoli, nā loli pH kūloko o nā cell i hoʻopili ʻia i nā mea ʻike.