Nā huahana

He kuleana koʻikoʻi ka Cysteine ​​​​(Cys) i nā kaʻina physiological he nui o nā cell eukaryotic a he mea koʻikoʻi kona ʻike ʻana i nā cell.Eia nō naʻe, ʻo ka glutathione (GSH), homocysteine, N-acetyl-L-cysteine ​​​​a me nā thiols ʻē aʻe e pale nui i ka ʻike ʻana iā Cys.Ma keʻano nui, keʻakeʻa nui neiʻo GSH i kaʻikeʻana i nā cellular Cys (30-200 μM) ma muli o kona kiʻekiʻe intracellular concentration (1-10 mM).Ma kēia hana, ua hōʻike ʻia kahi ʻimi fluorescent off-on (HOTA) no ka ʻike ʻana iā Cys.Loaʻa i kēia probe ka ʻike maikaʻi loa a me ka koho ʻoluʻolu no ka ʻike ʻana o Cys cellular: me ka hoʻohui ʻana o 200 μM Cys, ua hoʻonui ʻia ka ikaika fluorescence o ka probe (10 μM) i 117-fold a ua helu ʻia ka palena ʻike he 13.47 μM, ʻoi aku ka haʻahaʻa. ʻoi aku ka nui o ka helu Cys cellular;Ua ʻike ʻia e ka probe ka 30-200 μM cysteine ​​ma luna o 1-10 mM glutathione.No laila, ua hana ʻia nā hoʻokolohua kiʻi kelepona me ka HOTA probe.Eia kekahi, ua hōʻoia nā hopena o ka thiol-blocki ng a me GSH synthesis inhibiting hoʻokolohua ʻo ka hoʻokuʻu intracellular ka mea nui mai ka pilina ma waena o Cys a me HOTA.