X-GAL CAS:7240-90-6 98% Keʻokeʻo a i ke keʻokeʻo Crystalline Powder
| Helu Helu | XD90008 |
| inoa mea kūʻai | X-Gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) |
| CAS | 7240-90-6 |
| ʻĀpana Molekala | C14H15BrClNO6 |
| Kaumaha Molecular | 408.63 |
| Nā kikoʻī mālama | -2 a -6 °C |
| Kānāwai Kūʻai Kūlike | 29400000 |
Huahana Huahana
| ʻIke ʻia o ka Haʻina | Akaka, waihoʻoluʻu ʻole i ka wai melemele māmā (50mg/ml i DMF:MeOH, 1:1) |
| ʻO ka hoʻololi ʻana i ka optical kikoʻī | -61.5 +/- 1 |
| Ka nana aku | Keʻokeʻo a keʻokeʻo keʻokeʻo crystalline pauda |
| HPLC maemae | min 99% |
| Solubility (5 % ma DMF) | Hiki ke hoʻoheheʻe ʻia (5% w/v, DMF) |
| Wai KF | kiʻekiʻe 1% |
| ʻIke (HPLC ma ke kumu Anhydrous) | min 98% w/w |
Hoʻohana i ka X-gal
ʻO X-gal (hoʻopau ʻia ʻo BCIG no 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) he mea hoʻohuihui olaola me ka galactose i hoʻopili ʻia me kahi indole i pani ʻia.Ua hoʻopili ʻia ka pūhui e Jerome Horwitz a me nā hoa hana i ka makahiki 1964. Hoʻopōkole pinepine ʻia ka inoa kemika maʻamau i nā huaʻōlelo liʻiliʻi a liʻiliʻi hoʻi e like me ka bromochloroindoxyl galactoside.ʻO ka X mai ka indoxyl paha ke kumu o ka X i ka ʻokiʻoki X-gal.Hoʻohana pinepine ʻia ʻo X-gal i loko o ka biology molekula e hoʻāʻo ai i ka loaʻa ʻana o kahi enzyme, β-galactosidase, ma kahi o kāna pahuhopu maʻamau, he β-galactoside.Hoʻohana ʻia ia e ʻike i ka hana o kēia enzyme i ka histochemistry a me ka bacteriology.ʻO X-gal kekahi o nā indoxyl glycosides a me nā esters e hāʻawi mai i nā pūhui polū hiki ʻole ke hoʻoheheʻe ʻia e like me ka waiʻona indigo ma muli o ka hydrolysis i hoʻopaʻa ʻia e ka enzyme-catalyzed.
He analike ka X-gal o ka lactose, a no laila hiki ke hoʻoheheʻe ʻia e ka enzyme β-galactosidase nāna e hoʻokaʻawale i ka paʻa β-glycosidic i D-lactose.X-gal, i ka wā i hoʻokaʻawale ʻia e β-galactosidase, e hāʻawi mai i ka galactose a me ka 5-bromo- 4-chloro-3-hydroxyindole - 1. ʻO ka hope a laila hoʻomāliʻi wale ʻia a ua oxidized i 5,5'-dibromo-4,4'-dichloro. -indigo - 2, he hua polū polū ʻaʻole hiki ke hoʻoheheʻe ʻia.ʻAʻole kala ʻole ʻo X-gal ponoʻī, no laila hiki ke hoʻohana ʻia ka loaʻa ʻana o ka huahana ʻulaʻula i mea e hoʻāʻo ai no ka loaʻa ʻana o ka β-galactosidase ikaika.ʻAe kēia i ka β-galactosidase bacteria (i kapa ʻia ʻo lacZ ) e hoʻohana ʻia ma ke ʻano he mea hoʻolaha i nā noi like ʻole.
I loko o ʻelua-hybrid analysis, hiki ke hoʻohana ʻia ka β-galactosidase ma ke ʻano he mea hoʻolaha e ʻike ai i nā protein e pili ana kekahi i kekahi.Ma kēia ʻano, hiki ke nānā ʻia nā hale waihona puke genome no ka launa pū ʻana me ka hoʻohana ʻana i ka hū a i ʻole ka ʻōnaehana bacteria.Ma kahi e kūleʻa ai ka pilina ma waena o nā protein e nānā ʻia, e hopena ia i ka hoʻopaʻa ʻana i kahi kikowaena hoʻāla i kahi mea hoʻolaha.Inā pili ka mea hoʻolaha i kahi gene lacZ, ʻo ka hana ʻana o ka β-galactosidase, ka hopena i ka hoʻokumu ʻia ʻana o nā kolone uliuli-pigmented i mua o X-gal, no laila e hōʻike i kahi pilina kūleʻa ma waena o nā protein.Hiki ke kaupalena ʻia kēia ʻenehana i ka nānā ʻana i nā hale waihona puke ʻoi aku ka liʻiliʻi ma mua o 106. ʻO ka hoʻokaʻawale kūleʻa o X-gal e hana pū kekahi i kahi ʻala ʻino loa ma muli o ka volatilization o indole.
No ka mea he kala ʻole ʻo X-gal ponoʻī, hiki ke hoʻohana ʻia ka loaʻa ʻana o ka huahana ʻulaʻula i mea hoʻāʻo no ka loaʻa ʻana o kahi β-galactosidase ikaika.
ʻO kēia ʻike maʻalahi o kahi enzyme ikaika e hiki ai ke hoʻohana ʻia ka gene no βgalactosidase (ka lacZ gene) ma ke ʻano he moʻo nūhou i nā noi like ʻole.
