γ-L-Glutamyl-3-carboxy-4-nitroanilide, paʻakai monoammonium Cas:63699-78-5 ʻO ka pauka microcrystalline melemele māmā
| Helu Helu | XD90183 |
| inoa mea kūʻai | γ-L-Glutamyl-3-carboxy-4-nitroanilide, paʻakai monoammonium |
| CAS | 63699-78-5 |
| ʻĀpana Molekala | C12H12N3O7-.H4N+ |
| Kaumaha Molecular | 328.27 |
| Nā kikoʻī mālama | Ambient |
Huahana Huahana
| Ka nana aku | ʻO ka pauka microcrystalline melemele māmā |
| ʻO Assay | 99% |
| Lae hehee | 187ºC ± 2.0ºC |
| Lae paila | 718.3°C ma 760 mmHg |
| Kuhikuhi uila | 368.5 °C |
| Ka hikiwawe | H2O: 100 mg/mL, akaka, melemele-'ōmaʻomaʻo |
Ua noiʻi ʻia nā kinetics o ke kanaka serum gamma-glutamyltransferase (EC 2.3.2.2), me ka hoʻohana ʻana i ka glycylglycine ma ke ʻano he gamma-glutamyl acceptor substrate a me ka gamma-glutamyl-4-nitroanilide a me kāna derivative carboxy, gamma-glutamyl-3-carboxy-4 -nitroanilide, e like me nā substrate hāʻawi.Ua hoʻokumu ʻia ka hanana like ʻana o ka gamma-glutamyltransfer a me ka autotransfer ma ka iho ʻana o ka chromatography pepa.Hoʻopaʻa ʻia nā pānaʻi pānaʻi pālua like ʻole ʻaʻole pono ka mīkini enzyme (ping-pong bi-bi).ʻAʻole i ʻike ʻia ka pale ʻana e kekahi mea hāʻawi, a ʻike ʻia ka pale ʻana e ka glycylglycine ma nā ʻano ʻoi aku ma mua o nā mea hoihoi.Ua hoʻohana ʻia nā ʻikepili kinetic i loaʻa ma ka loiloi regression nonlinear o ka ʻikepili wikiwiki mua no ka hoʻoholo ʻana i nā koina substrate reagent no ka hoʻāʻo ʻana o kēia enzyme.ʻO kahi hoʻāʻo me ka hoʻohana ʻana i 4 mmol o gamma-glutamyl-3-carboxy-4-nitroanilide a me 100 mmol o glycylglycine no ka lita i loaʻa i nā hana like i kēlā me ka hoʻohana ʻana i ka 4 mmol o gamma-glutamyl-4-nitroanilide a me 40 mmol o glycylglycine no ka lita.ʻO kēia mau manaʻo o ka mea hāʻawi carboxy a me ka glycylglycine he "kūʻai maikaʻi loa" a ʻaʻohe pilikia o ke kaʻina hana ke hoʻohana ʻia.
