ʻO ka maʻi maʻi entomopathogenic, Xenorhabdus nematophila, hoʻoulu i ka immunosuppression o nā pepeke i hoʻopaʻa ʻia ma ke kāohi ʻana i ka hana phospholipase A(2) (PLA(2)).I kēia mau lā, ua ʻike ʻia kahi moʻo PLA(2) pili i ka immune mai ka namu palaoa ʻulaʻula, ʻo Tribolium castaneum.Hoʻopili kēia haʻawina i kēia gene PLA(2) i loko o kahi vector hōʻike bacterial e hana i kahi enzyme recombinant.Ua hōʻike ka recombinant T. castaneum PLA(2) (TcPLA(2)) i kona ʻano hana enzyme me ka hoʻopaʻa ʻana o ka substrate, ka pH, a me ka mahana ambient.Ua kūlike kona mau hiʻohiʻona biochemical i kahi ʻano huna o PLA(2) (sPLA(2)) no ka mea, ua pāpā ʻia kāna hana e ka dithiothreitol (he mea hoʻohaʻahaʻa o ka paʻa disulfide) a me ka bromophenacyl bromide (kahi mea hoʻopaneʻe sPLA (2) kikoʻī) akā ʻaʻole e ka methylarachidonyl. fluorophosphonate (kahi ʻano cytosolic kikoʻī o PLA (2)).Aia i loko o ka X. nematophila culture broth i loko o ka PLA(2) inhibitory factor(s), ka mea i nui loa i loko o ka media i loaʻa ma kahi pae ulu hua bacteria paʻa.ʻO ka PLA(2) mea hoʻohilahila (s) he pale wela a unuhi ʻia i loko o nā hakina wai a me nā ʻāpana.Ua like like ka hopena o kahi hakina PLA(2)-inhibitory i ka immunosuppression o T. castaneum me ka hopena o ke kaohi ʻana i ka hōʻike gene TcPLA(2) e ka RNA interference.
