He monoclonal antibody (mAb 8281) kiko'ī no ka terminal β-galactose (βGal) o glycosphingolipids (GSL) a me glycoproteins i hana ʻia mai nā ʻiole i hoʻopaʻa ʻia me ka lipid extract mai nā pūnaewele lymphocytic leukemia hou (ALL).Immuno-thin layer chromatography (ITLC) a me nā hoʻokūkū hoʻokūkū me nā maʻamau GSL kūʻokoʻa i hoʻomaʻemaʻe ʻia, nā sugars manuahi, a me nā neoglycoproteins synthetic i hōʻike i ka mAb 8281 e hoʻoikaika ikaika me LacCer, GalCer a me Gal-β-O-(CH3)2S(CH3)2- Hoʻopili ʻia ʻo CONH-(Gal-β-O-CETE) i ka bovine serum albumin (BSA).ʻO ke kō penultimate kekahi i hana i ka hoʻopaʻa ʻana.ʻAʻole hoʻoikaika ka antibody me nā kaʻapona me nā hale αGal hope a me nā ʻāpana hope pili ʻole.Ua hōʻike ʻia ka ʻili ʻana o ka immunoperoxidase indirect a me ke kahe ʻana o ka cytometry me ka mAb 8281 i ka ʻili maikaʻi ma nā ʻiʻo he nui, e like me ka ʻiʻo maʻemaʻe, ka mucosa gastrointestinal, nā pūnāwai lymph node B a me nā monocytes.ʻO ka loiloi ITLC o ka GSL o nā neoplasms cell B hou me ka mAb 8281 i hōʻoia i ka hiki ʻana o ka lactosylceramide a me ka galactosylceramide i nā neoplasms o nā pae like ʻole o ka ʻokoʻa.Ma muli o kona kiko'ī no nā koena carbohydrate βGal hope loa, hiki i ka mAb 8281 ke hoʻohana i nā loiloi hana a me ka hana o GSL.
