ONPG CAS:369-07-3 98.0% Min Keʻokeʻo a i ʻole -Papa Keʻokeʻo
| Helu Helu | XD90006 |
| CAS | 369-07-3 |
| inoa mea kūʻai | ONPG(2-Nitrophenyl-beta-D-galactopyranoside) |
| ʻĀpana Molekala | C12H15NO8 |
| Kaumaha Molecular | 301.25 |
| Nā kikoʻī mālama | 2 a 8 °C |
| Kānāwai Kūʻai Kūlike | 29400000 |
Huahana Huahana
| Maʻemaʻe (HPLC) | Min.98.0% |
| Ka nana aku | Keʻokeʻo a keʻokeʻo - pauda keʻokeʻo |
| Hoʻoholo(1% i ka wai) | ʻAkaka, waihoʻoluʻu ʻole a i ʻole melemele iki |
| Waiwai(Karl Fisher) | Max.0.5% |
| ʻO ka hoʻololi ʻike kikoʻī [α]D20(c=1, H2O) | - 65.0 ° C a i -73.0 ° C |
Kūkākūkā ma ka hoʻāʻo ONPG (hōʻike β-galactosidase)
Ua hāpai pinepine ʻia nā nīnau i kēia mau lā: 1. No ke aha e hiki ai ke hoʻohana ʻia ka hoʻāʻo ONPG e ʻike i ka fermentation lactose lohi?2. No ke aha i ʻōlelo ai ka maʻamau o ka ʻāina he pono ke hoʻohana i ka 3% sodium chloride trisaccharide hao (a i ʻole trisaccharide hao) no ka hoʻāʻo ONPG?3. No Vibrio parahaemolyticus, i ka wā e ho'āʻo ai i ka OPNG, no ke aha e hoʻohui ʻia ai ka toluene i ka dropwise e like me ke kūlana?He aha ka hana?
Ua loiloi kā mākou hui i nā ʻike he nui, hōʻuluʻulu ʻia, a kaʻana like iā ʻoe ma lalo nei:
Kumu: ʻO ka inoa Kina o ONPG he o-nitrobenzene-β-D-galactopyranoside.Hiki ke hoʻokahe ʻia ka ONPG i ka galactose a me ka o-nitrophenol melemele (ONP) e β-galactosidase, no laila hiki ke ʻike ʻia ka hana o ka β-galactosidase e ka hoʻololi kala o ka moʻomeheu.
ʻO ka Lactose kahi kō e pono ai ka hapa nui o nā microorganisms e ʻike.Pono kona metabolism i ʻelua enzymes, hoʻokahi ka cell permease, komo ka lactose i nā cell ma lalo o ka hana o ka permease;ʻo ka β-galactosidase ka mea ʻē aʻe, nāna e hoʻokahe i ka lactose i galactose.ʻO ka lactose a me ka glucose.Hiki i ka β-Galactosidase ke hana pololei i ka ONPG e hoʻokahe i ka galactose a me ka o-nitrophenol melemele (ONP).Hiki ke hana i loko o 24h, me ka lactose hoʻopaneʻe fermenters.No laila, wehewehe ʻo ia i nā hopena ʻike o ke ʻohi ʻana i ka moʻomeheu 1 mai ka slant agar a hoʻopaʻa ʻia i loko o ka pōʻai piha ma 36°C no 1-3h a me 24h.Inā hana ʻia ka β-galactosidase, e lilo ia i melemele i ka hola 1-3, inā ʻaʻohe enzyme e like me ia, ʻaʻole ia e hoʻololi i ke kala i 24h.
Wahi a nā enzyme ʻelua i luna, hiki ke hoʻokaʻawale ʻia nā microorganism i nā ʻāpana aʻe:
1 lactose-fermenting (18-24 hola) bacteria me ka permease a me ka β-galactosidase P + G +;
2 Hoʻopaneʻe ka lactose fermenters (ʻoi aku ka lōʻihi ma mua o 24 mau hola) nele i ka permease akā loaʻa ka galactosidase: P-G+.
3 ʻAʻohe lactose fermenters nele i ka permease a me ka galactosidase: P- G-.
Hiki ke hoʻohana ʻia ka hoʻāʻo ONPG e hoʻokaʻawale i ka bacteria lactose-lag-fermenting bacteria (PG+) mai ka bacteria lactose non-fermenting (PG-), e like me:
1 E hoʻokaʻawale i nā mea hoʻoheheʻe lactose hope (P- G+) mai nā mea hoʻoheheʻe lactose ʻole (P- G-).
(a) Citrobacter (+) a me Salmonella arizonae (+) mai Salmonella (-).
(b) Escherichia coli (+) mai Shigella sonnei (-).
No ke aha i hana ʻia ai ka hōʻike ONPG me ka hoʻohana ʻana i ka moʻomeheu i ka pō ma 3% sodium chloride ferric trisaccharide (iron trisaccharide)?Ua kūkākūkā kā mākou hui i nā ʻike he nui, akā ʻaʻohe ʻōlelo maopopo.Ma ka pūnaewele FDA wale nō, ua kākau ʻia "E hoʻāʻo ʻia nā moʻomeheu Inoculate ma ke kolu kolu iron agar slants a incubate no 18 mau hola ma 37 ° C (a i ʻole ka wela kūpono, inā makemake ʻia). Hiki ke hoʻohana ʻia ka % lactose."'o ia ho'i: ua ho'ā 'ia ka bacteria ho'ā'o ma luna o ka mea hao trisaccharide a ho'oulu 'ia ma 37°C no 18h.Hiki ke ʻae ʻia nā meaʻai agar slants (a i ʻole) me ka 1% lactose.No laila, ua manaʻo ʻia he lactose ka mea hao trisaccharide.Ma hope o ka ulu ʻana o ka pō, ua hoʻopuka ka bacteria i kahi β-galactosidase ikaika maikaʻi.Me ka hoʻohana ʻana i ia mau bacteria, hiki ke hoʻoheheʻe ʻia ka ONPG e β-galactosidase wikiwiki.ʻOi aku ka wikiwiki a hōʻike maikaʻi ʻia ka hanana hoʻokolohua.Eia kekahi, ʻo ka hoʻohui dropwise o toluene a me ka ʻauʻau wai no 5 mau minuke i mea e hoʻokuʻu piha ai i ka β-galactosidase.
