Hoʻolaha ākea nā protein processor hōʻailona PII i nā prokaryotes a me nā mea kanu kahi e mālama ai lākou i ka nui o nā hopena anabolic.Pono ka hoʻonui ʻana o nā metabolites i ka hoʻomaha ʻana i nā kaʻa kaʻa hoʻokele paʻa.Ma aneʻi mākou e hōʻike nei i ka hoʻololi ʻana o ka helu hoʻokahi i ka protein hōʻailona PII mai ka cyanobacterium Synechocystis sp.Ua lawa ka PCC 6803 e wehe i ke ala arginine e hoʻonui ai i ka biopolymer cyanophycin (multi-L-arginyl-poly-L-aspartate).ʻO kēia huahana he hoihoi biotechnological ma ke ʻano he kumu o nā amino acids a me ka polyaspartic acid.Hōʻike kēia hana i kahi ala hou o ka ʻenekinia alahele ma o ka hoʻolālā ʻana i nā protein hōʻailona PII i hana maʻamau.Ma ʻaneʻi, ʻo Synechocystis sp.PCC6803 strain me ka PII-I86N mutation over-accumulated arginine ma o ka constitutive activation o ka enzyme kī N-acetylglutamate kinase (NAGK). ma mua o ke ano hihiu.ʻO ka hopena, ua hōʻiliʻili ʻia ke kānana BW86 a hiki i ka 57 % cyanophycin no kēlā me kēia cell dry mass ma lalo o nā kūlana i hoʻāʻo ʻia, ʻo ia ka hua kiʻekiʻe o ka cyanophycin i hōʻike ʻia a hiki i kēia lā.Hoʻopuka ʻo Strain BW86 i ka cyanophycin i loko o kahi ʻano nui o ka molekala o 25 a >100 kDa;ʻO ka ʻano ʻāhiu i hana i ka polymer ma kahi o 30 a > 100 kDa. ʻO ka hua kiʻekiʻe a me ka nui mole kiʻekiʻe o ka cyanophycin i hana ʻia e ka strain BW86 me nā koi haʻahaʻa haʻahaʻa o ka cyanobacteria e lilo ia i mea hoʻohiki no ka hana biotechnological o cyanophycin.Hōʻike hou kēia haʻawina i ka hiki ke hoʻohana ʻia i ka ʻenekinia ala metabolic me ka hoʻohana ʻana i ka protein hōʻailona PII, i loaʻa i nā maʻi bacteria he nui.
