Nā huahana

Hoʻoponopono ka protein phosphorylation i nā hana cellular like ʻole a he hana koʻikoʻi i ka hoʻomohala mua ʻana o nā mea kanu.No ka hoʻokō a hoʻonui ʻana i nā noiʻi mua o ka phosphorylation protein i nā mea kanu Arabidopsis ua hoʻohana mākou i kahi ala ʻē aʻe e hui pū ai i ka ʻili ʻana o ka protein ma lalo o nā kūlana non-denaturing me ka immobilized metal-ion affinity chromatography (IMAC) hoʻonui i nā phosphoproteins intact in Rubisco-depleted extracts, a ukali ʻia e ka ʻike. me ka hoʻohana ʻana i ka electrophoresis gel ʻelua-dimensional (2-DE) a me ka chromatography-tandem mass spectrometry (LC-MS/MS).In-gel trypsin digestion a me ka nānā ʻana o nā wahi gel i koho ʻia he 144 phosphorylated peptides a me nā koena, nona wale nō 18 phosphopeptides a me 8 phosphosite i loaʻa i ka PhosPhAt 4.0 a me P3DB Arabidopsis thaliana phosphorylation site database.ʻOi aku ma mua o ka hapalua o nā phosphoproteins 82 i ʻike ʻia i loko o ka metabolism carbohydrate, photosynthesis/respiration a i ʻole oxidative stress pane mechani sms.ʻO ka hoʻonui ʻia ʻana o nā phosphoproteins intact ma mua o 2-DE a me LC-MS/MS e hoʻonui i ka ʻike ʻana o ka threonine phosphorylated a me nā koena tyrosine i hoʻohālikelike ʻia me nā ʻano e hoʻohana ai i ka hoʻonui ʻana i ka pae peptide, e hōʻike ana he ʻano hoʻohui ʻia nā ala ʻelua i ke ʻano o ka uhi pūnaewele phosphorylation.ʻO ka hoʻohālikelike ʻana i nā hopena no nā mea kanu ʻōpio me nā mea i loaʻa mua no nā lau Arabidopsis oʻo i ʻike ʻia ʻelima mau protein i hoʻokaʻawale ʻia i ka phosphorylated i loko o kēia mau kiko, e hōʻike ana i ka hiki o kēia ʻenehana no ka noiʻi ʻana i ka dinamika o ka phosphorylation protein i ka wā o ka ulu ʻana o nā mea kanu.